Cellular uptake and imaging studies of gadolinium-loaded single-walled carbon nanotubes as MRI contrast agents
Cellular uptake and imaging studies of gadolinium-loaded single-walled carbon nanotubes as MRI contrast agents
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DOI：
10.1002/cmmi.410
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发表时间：
2011-03-01
影响因子：
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通讯作者：
Wong, Kelvin K.
中科院分区：
文献类型：
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作者：
Tang, Annie M.;Ananta, Jeyarama S.;Wong, Kelvin K.
我们首次使用体外 R-2 和 R-2* 映射在 3 T MRI 扫描仪中量化钆负载超短单壁碳纳米管（钆纳米管或 GNT）的细胞内摄取（J774A.1 鼠巨噬细胞）。随着细胞浓度的增加，GNT 标记的细胞表现出净横向弛豫（Delta R-2 和 Delta R-2*）的高线性变化。对于每个细胞浓度，测得的 Delta R-2* 大约是相应 Delta R-2 的三到四倍。 GNT 的细胞内摄取通过电感耦合等离子体发射光谱 (ICP-OES) 进行验证，表明每个细胞的平均细胞摄取量为 0.44 +/- 0.09 pg Gd 或每个细胞 1.69 X 10(9) Gd3+ 离子。细胞增殖 MTS 测定表明，细胞被有效标记，且 GNT 浓度无细胞毒性
We quantify here, for the first time, the intracellular uptake (J774A.1 murine macrophage cells) of gadolinium-loaded ultra-short single-walled carbon nanotubes (gadonanotubes or GNTs) in a 3 T MRI scanner using R-2 and R-2* mapping in vitro. GNT-labeled cells exhibited high and linear changes in net transverse relaxations (Delta R-2 and Delta R-2*) with increasing cell concentration. The measured Delta R-2* were about three to four times greater than the respective Delta R-2 for each cell concentration. The intracellular uptake of GNTs was validated with inductively coupled plasma optical emission spectrometry (ICP-OES), indicating an average cellular uptake of 0.44 +/- 0.09 pg Gd per cell or 1.69 X 10(9) Gd3+ ions per cell. Cell proliferation MTS assays demonstrated that the cells were effectively labeled, without cytotoxicity, for GNTs concentrations