ST2 suppresses IL-6 production via the inhibition of IκB degradation induced by the LPS signal in THP-1 cells
ST2 suppresses IL-6 production via the inhibition of IκB degradation induced by the LPS signal in THP-1 cells
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DOI：
10.1016/j.bbrc.2005.12.206
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发表时间：
2006-03-10
影响因子：
3.1
通讯作者：
Tominaga, S
中科院分区：
文献类型：
--
作者：
Takezako, N;Hayakawa, M;Tominaga, S
LPS通过刺激Toll样受体诱导炎性细胞因子的产生。在这项研究中，我们证明了可溶性分泌形式的ST 2基因产物（M），白细胞介素-1受体家族的I成员，抑制了LPS刺激的人单核细胞白血病细胞系THP-1中IL-6的产生。免疫荧光共聚焦显微镜显示ST 2与THP-1细胞表面的结合，其中ST 2导致核因子-κ B与IL-6启动子的结合减少。此外，ST 2预处理可减少LPS刺激后细胞质中1 KB的降解。这些结果表明，ST 2通过抑制THP-1细胞中1 KB降解来负调节LPS诱导的IL-6产生。(c)2006年爱思唯尔公司All rights reserved.
LPS induces the production of inflammatory cytokines via the stimulation of Toll-like receptors. In this study, we demonstrated that a Soluble secreted form of the ST2 gene product (M), I member of the interleukin-1 receptor Family, suppressed the production of IL-6 in an LPS-stimulated human monocytic leukemia cell line, THP-1. Immunofluorescence confocal microscopy revealed the binding of ST2 to the surface of the THP-1 cells, in which ST2 led to decreased binding of nuclear factor-kappa B to the IL-6 promoter. Furthermore, the degradation of 1KB in the cytoplasm after LPS stimulation was reduced by pretreatment with ST2. These results demonstrated that ST2 negatively regulates LPS-induced IL-6 production via the inhibition of 1KB degradation in THP-1 cells. (c) 2006 Elsevier Inc. All rights reserved.