Immunocytochemical localization of neuron specific enolase in small cell carcinomas and carcinoid tumours of the lung
Immunocytochemical localization of neuron specific enolase in small cell carcinomas and carcinoid tumours of the lung
复制标题
肺小细胞癌和类癌肿瘤中神经元特异性烯醇化酶的免疫细胞化学定位
作者：
M. Sheppard;B. Corrin;M. Bennett;P. Marangos;S. R. Bloom;J. Polak
肺类癌和小细胞癌与正常神经内分泌细胞有许多共同的特征。虽然类癌含有许多致密核心的神经分泌颗粒，并且经常是嗜银的，但小细胞癌颗粒性差，很少嗜银，这使人怀疑它们的神经内分泌性质。神经元特异性烯醇化酶（NSE）的免疫染色最近被用于显示肺的神经内分泌成分，包括神经和神经内分泌细胞。因此，我们使用NSE免疫染色研究79个肺肿瘤，包括18支气管类癌和31个小细胞癌的神经内分泌分化，并将这些结果与银染色结果进行比较。18个类癌中有13个对银有反应，所有其他类型均为阴性。NSE免疫反应发生在16个类癌和18个小细胞癌中。检查的鳞状细胞癌、大细胞未分化癌和腺癌均未显示NSE免疫反应性。从10个新鲜的肺肿瘤中提取的NSE放射免疫分析与免疫染色结果相关性很好，表明在两个小细胞癌（334和517纳克/毫克蛋白质）和三个类癌（152，908和1143纳克/毫克蛋白质）大量。4例鳞状细胞癌（31-44 ng/mg蛋白质）和1例大细胞间变性癌（30 ng/mg蛋白质）的值要低得多，这是由于周围肺中存在NSE阳性神经和神经内分泌细胞所致。NSE免疫染色是肺肿瘤神经内分泌分化的一个有用的标志物，在小细胞间变性肿瘤及其转移的诊断中应证明特别有价值。
Carcinoid tumours and small cell carcinomas of the lung share many characteristics with normal neuroendocrine cells. While carcinoid tumours contain many dense‐cored neurosecretory granules and are frequently argyrophil, small cell carcinomas are poorly granulated and rarely argyrophil, which casts doubt on their neuroendocrine nature. Immunostaining of the enzyme neuron specific enolase (NSE) was recently used to demonstrate the neuroendocrine components of the lung including nerves and neuroendocrine cells. We therefore used NSE immunostaining to investigate neuroendocrine differentiation in 79 lung tumours, including 18 bronchial carcinoids and 31 small cell carcinomas, and compared these results with those obtained with silver stains. Thirteen of the 18 carcinoids were reactive to silver, all other types being negative. NSE‐immunoreactivity occurred in 16 carcinoids and 18 small cell carcinomas. None of the squamous cell carcinomas, large cell anaplastic carcinomas and adenocarcinomas examined showed NSE‐immucoreactivity. Radioimmunoassay of extractable NSE from 10 fresh lung tumours correlated well with the immunostaining results, demonstrating large amounts in two small cell carcinomas (334 and 517 ng/mg protein) and three carcinoids (152, 908, and 1143 ng/mg protein). Values were much lower for four squamous cell carcinomas (31–44 ng/mg protein) and one large cell anaplastic carcinoma (30 ng/mg protein) and were accounted for by the presence of NSE‐positive nerves and neuroendocrine cells in the surrounding lung. NSE immunostaining is a useful marker of neuroendocrine differentiation in lung tumours and should prove particularly valuable in the diagnosis of small cell anaplastic tumours and their metastases.