Molecular cloning and expression of a chloride ion channel of cell nuclei
Molecular cloning and expression of a chloride ion channel of cell nuclei
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DOI：
10.1074/jbc.272.19.12575
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发表时间：
1997-05-09
影响因子：
4.8
通讯作者：
Breit, SN
中科院分区：
文献类型：
--
作者：
Valenzuela, SM;Martin, DK;Breit, SN
已知离子通道存在于几乎所有细胞的质膜上，并在各种细胞内细胞器的膜上被发现。然而，直到最近，它们还被认为不存在于核膜上。在这项研究中，我们描述了人单核细胞系U937核离子通道蛋白的分子克隆和鉴定，命名为核氯离子通道-27(NCC27)。NCC27是一种新的氯离子通道蛋白，主要定位于细胞核，其唯一已知的同源物是牛氯离子通道蛋白(P64)，据信定位于内部细胞器。因此，NCC27代表了一类新的有机氯离子通道蛋白的第一个人类成员。
Ion channels are known to be present on the plasma membrane of virtually all cells and have been found on the membranes of various intracellular organelles. However, until recently they were believed not to occur at the nuclear membrane. In this study we describe the molecular cloning and characterization of a nuclear ion channel protein, designated nuclear chloride channel-27 (NCC27), from the human myelomonocytic cell line, U937. NCC27 is a novel chloride ion channel protein that was found to localize principally to the cell nucleus, Its only known homologue is a bovine chloride ion channel protein (p64) believed to localize to internal organelles. NCC27 therefore represents the first human member of a new class of organellar chloride ion channel proteins.