Decreased 5-Hydroxymethylcytosine (5-hmC) predicts poor prognosis in early-stage laryngeal squamous cell carcinoma.
Decreased 5-Hydroxymethylcytosine (5-hmC) predicts poor prognosis in early-stage laryngeal squamous cell carcinoma.
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5-羟甲基胞嘧啶 (5-hmC) 减少预示早期喉鳞状细胞癌的预后不良。
DOI：
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发表时间：
2016-05
影响因子：
5.3
通讯作者：
Ji Meiju
中科院分区：
文献类型：
--
作者：
Zhang Yanfang;Wu Kexia;Shao Yuan;Sui Fang;Yang Qi;Shi BIngyin;Hou Peng;Ji Meiju
越来越多的证据表明，5-羟甲基胞嘧啶（5-hmC）的大规模丢失是不同癌症的表观遗传学标志。然而，喉鳞状细胞癌（LSCC）中5-hmC的水平及其在这种癌症的预后价值仍然在很大程度上未知。本研究采用斑点杂交和定量RT-PCR方法检测5-hmC在喉鳞癌中的表达水平及泰特-1、-2和-3的表达，探讨5-hmC与喉鳞癌临床病理特征和临床预后的关系。我们的数据表明，5-hmC显着降低，与匹配的正常组织相比，在LSCC。我们还发现5-hmC的降低与泰特-1基因表达的降低之间有很强的联系，但与泰特-2或-3无关，这表明泰特-1表达的降低与喉鳞状细胞癌中5-hmC的丢失有关。此外，Mann-Whitney U检验显示5-hmC含量与吸烟（P = 0.039）和肿瘤侵袭（P = 0.004）显著相关。重要的是，我们发现5-hmC的降低与早期喉鳞癌患者的生存率低显著相关（P = 0.043）。总之，我们的研究结果表明，可能由泰特-1减少引起的5-hmC降低在喉鳞癌的临床病理学中是至关重要的，并且是早期喉鳞癌患者预后不良的因素。
Accumulating evidences suggest that large-scale loss of 5-hydroxymethylcytosine (5-hmC) is an epigenetic hallmark in different cancers. However, the levels of 5-hmC in laryngeal squamous cell carcinoma (LSCC) and its prognostic value in this cancer remain largely unknown. Using dot-blot and quantitative RT-PCR assays, we investigate 5-hmC levels and expression of TET-1, -2 and -3 in LSCCs and explore the association of 5-hmC levels with clinicopathological characteristics and clinical outcome of LSCC patients. Our data showed that 5-hmC was significantly decreased in LSCCs as compared with matched normal tissues. We also found a strong link between decreased 5-hmC and the reduction of TET-1 gene expression, but not TET-2 or -3, suggesting that decreased TET-1 expression was implicated in 5-hmC loss in LSCC. Moreover, Mann-Whitney U tests showed that 5-hmC content was significantly associated with smoking (P = 0.039) and tumor invasion (P = 0.004). Importantly, we found that decreased 5-hmC was significantly associated with poor survival of early-stage LSCC patients (P = 0.043). Altogether, our findings implicate that decreased 5-hmC probaly caused by the reduction of TET-1 is crucial to the clinical pathology of LSCC and is a poor prognostic factor in ealry-stage LSCC patients.
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影响因子：
30.5
作者：
Cimmino L;Dawlaty MM;Ndiaye-Lobry D;Yap YS;Bakogianni S;Yu Y;Bhattacharyya S;Shaknovich R;Geng H;Lobry C;Mullenders J;King B;Trimarchi T;Aranda-Orgilles B;Liu C;Shen S;Verma AK;Jaenisch R;Aifantis I
通讯作者：
Aifantis I
DOI：
10.1056/nejmc091348
发表时间：
2009-09
期刊：
The New England journal of medicine
影响因子：
--
作者：
A. Tefferi;Ken-Hong Lim;R. Levine
通讯作者：
A. Tefferi;Ken-Hong Lim;R. Levine
影响因子：
4.8
作者：
Lopez, Fernando;Sampedro, Teresa;Alvarez-Marcos, Cesar
通讯作者：
Alvarez-Marcos, Cesar
影响因子：
16
作者：
Xu Y;Wu F;Tan L;Kong L;Xiong L;Deng J;Barbera AJ;Zheng L;Zhang H;Huang S;Min J;Nicholson T;Chen T;Xu G;Shi Y;Zhang K;Shi YG
通讯作者：
Shi YG
影响因子：
3.7
作者：
Liu C;Liu L;Chen X;Shen J;Shan J;Xu Y;Yang Z;Wu L;Xia F;Bie P;Cui Y;Bian XW;Qian C
通讯作者：
Qian C