Association between mossy fiber sprouting and expression of semaphorin-3f protein in dentate gyrus of hippocampus in lithium-pilocarpine-induced status epilepticus mouse model
Association between mossy fiber sprouting and expression of semaphorin-3f protein in dentate gyrus of hippocampus in lithium-pilocarpine-induced status epilepticus mouse model
复制标题
锂毛果芸香碱致癫痫持续状态小鼠模型苔藓纤维出芽与海马齿状回semaphorin-3f蛋白表达的关系
DOI：
10.1080/01616412.2016.1243639
复制
发表时间：
2016-01-01
影响因子：
1.9
通讯作者：
Zhou, Liemin
中科院分区：
文献类型：
--
作者：
Cai, Xiaodong;Long, Ling;Zhou, Liemin
目的：苔藓纤维发芽参与内侧颞叶癫痫的发病机制。但苔藓纤维发芽形成的确切机制尚不清楚。信号蛋白3f可抑制神经元轴突的生长。本研究的目的是评估信号素-3f表达与苔藓纤维发芽之间的关系。方法：首先建立匹罗卡品诱导的癫痫持续状态（PISE）模型。然后用Timm染色法检测PISE模型海马苔藓纤维发芽情况。western blot和免疫组化检测信号蛋白-3f的表达。研究了海马不同亚区信号蛋白3f的表达及其与苔藓纤维发芽的关系。结果：PISE组在齿状回（DG）区出现苔藓状纤维发芽。PISE组潜伏期开始发育，慢性期明显增高。DG区信号素-3f蛋白的表达在潜伏期开始下降，在慢性期维持在低水平。在其他组中没有发现这种变化。结论：上述结果表明，PISE模型中DG区信号素-3f表达的下降与苔藓纤维出芽的变化是平行的，提示PISE模型中苔藓纤维出芽与信号素-3f表达的降低密切相关。
Objectives: Mossy fiber sprouting is involved in the pathogenesis of mesial temporal lobe epilepsy. But the exact mechanism of formation of mossy fiber sprouting is still unclear. Semaphorin-3f protein could inhibit the growth of neuron axons. The aim of this research is to evaluate the association between semaphorin-3f expression and mossy fiber sprouting.Methods: We established pilocarpine-induced status epilepticus (PISE) models firstly. Then, mossy fiber sprouting in the hippocampus of PISE models was examined by Timm staining. Expression of semaphorin-3f was evaluated by western blot analysis and immunohistochemical examination. Expression of semaphorin-3f protein in different subregions of hippocampus and its relationship with mossy fiber sprouting were studied.Results: We found that in PISE group, mossy fiber sprouting appeared in dentate gyrus (DG) region. It started to develop in the latent phase of PISE group and increased significantly in the chronic phase. Expression of semaphorin-3f protein in DG region started to decrease in the latent phase, and stayed at low level in the chronic phase. No such change was found in the other groups.Conclusions: These results indicate that the decrease in semaphorin-3f expression in DG region was in parallel to the change of mossy fiber sprouting in PISE models, suggesting that mossy fiber sprouting is closely associated with reduced expression of semaphorin-3f in this model.