Olfactory classical conditioning in newborn mice
Olfactory classical conditioning in newborn mice
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DOI：
10.1016/j.bbr.2005.01.010
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发表时间：
2005-06-03
影响因子：
2.7
通讯作者：
Gallego, J
中科院分区：
文献类型：
--
作者：
Bouslama, M;Durand, E;Gallego, J
确定遗传改变小鼠的行为表型是阐明基因功能及其在认知障碍中的作用的有价值的方法。用于新生小鼠表型分型的方法很少，并且通常限于感觉运动反射。在这里，我们描述了一个简单的方法来评估新生小鼠的联想能力。我们使用了两种气味选择经典的条件反射模式的小鼠从出生之日（出生后年龄0，PO）到P6。采集需要20次试验：10次试验期间，将幼鼠放置在条件刺激（CS+）气味（柠檬或薄荷）上30 s，同时用画笔轻轻抚摸，10次试验期间，将幼鼠放置在其他气味（CS-）上30 s，不抚摸。然后，这些幼崽接受了五次气味偏好试验，以测试条件反射。在5小时和24小时后重复这五个试验的序列，以评估条件气味偏好的保留。在采集后立即进行的序列中，幼仔在CS+上花费的时间显著多于CS-（P < 0.0001）。在该测试期间未观察到条件偏好的消退。在5或24小时后没有观察到偏好，表明条件反应迅速丧失。预处理在P0-P1时有效。因此，条件反射可能比以前报道的更早出现在新生小鼠中。这种模式非常适合于对大样本的遗传改变小鼠进行表型分析，并可能揭示基因在儿科认知障碍中的作用。(c)2005 Elsevier B. V.保留所有权利。
Determining the behavioural phenotype of genetically altered mice is a valuable approach for elucidating the function of genes and their role in cognitive disorders. Methods for phenotyping newborn mice are scarce and generally confined to sensorimotor reflexes. Here, we describe a simple method for assessing associative abilities in newborn mice. We used a two-odour-choice classical conditioning paradigm in mice from the day of birth (post-natal age 0, PO) to P6. Acquisition required 20 trials: 10 trials during which the pups were placed over the conditioned stimulus (CS+) odour (lemon or peppermint) for 30 s and simultaneously stroked gently with a paintbrush and 10 trials during which the pups were placed over the other odour (CS-) for 30 s, without stroking. Then, the pups were subjected to five odour-preference trials to test for conditioning. This sequence of five trials was repeated after 5 and 24 h to assess retention of the conditioned odour preference. During the immediate post-acquisition sequence, the pups spent significantly more time over the CS+ than over the CS- (P < 0.0001). No extinction of the conditioned preference was observed during this test. No preference was observed after 5 or 24 h, indicating that the conditioned response was promptly lost. Conditioning was effective as soon as P0-P1. Thus, conditioning may emerge in newborn mice sooner than previously reported. This paradigm is well suited to phenotyping of large samples of genetically altered mice and may shed light on the role for genes in paediatric cognitive impairments. (c) 2005 Elsevier B.V. All rights reserved.