Identification of the Habitat of Radix Scrophulariae with Chromatographic Fingerprinting
Identification of the Habitat of Radix Scrophulariae with Chromatographic Fingerprinting
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发表时间：
2009
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通讯作者：
Sun Chan-juan
中科院分区：
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作者：
Sun Chan-juan
目的应用色谱指纹图谱技术对玄参药材进行鉴别。方法对10批玄参药材进行有效成分提取，采用高效液相色谱法，以Kromasil TM C18柱为色谱柱，以甲醇-1%冰醋酸为移动的相，检测波长290 nm，流速1.0 ml·min-1，柱温30℃。采用高效液相色谱法对重庆武隆玄参药材进行了评价，建立了玄参药材标准HPLC指纹图谱模型;以肉桂酸为对照峰，计算各特征峰的相对时间和相对峰面积比，利用该模型及特征成分的相对峰面积比可对浙江省的样品进行鉴别为玄参的规范种植和质量控制提供依据。
Objective To identify the Habital of Radix Scrophulariae with Chromatographic Fingerprinting.Methods Active constituents were extracted from ten batches of sample of Radix Scrophulariae(Scrophularia ningpoensis Hemsl.),which collected from Chongqing Wulong county,and evaluated by HPLC.Chromatographic conditions was Kromasil TM C18 column with methanol-1% glacial acetic acid as mobile phase,the detection wavelength was 290 nm,the flow rate was 1.0 ml·min-1,and column temperature was 30℃.Results Many active constituents that extracted from ten batches of sample of Radix Scrophulariae(Scrophularia ningpoensis Hemsl.),which collected from Chongqing Wulong county,were evaluated by HPLC.The models of standard HPLC Fingerprint of Radix Scrophulariae were established;Taking the cinnamic acid as the reference peak,the relative time and the relative peak area ration of various characteristics peak were calculated,and the sample from Zhejiang province could be distinguished by this model and the relative peak area ratio of the characteristic components.Conclusion This study is good for the standard planting and quality control of Radix scrophulariae.