Dysembryoplastic neuroeptihelial tumor (DNT): an immunohistochemical and ultrastructural study.
Dysembryoplastic neuroeptihelial tumor (DNT): an immunohistochemical and ultrastructural study.
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胚胎发育不良神经上皮肿瘤（DNT）：一项免疫组织化学和超微结构研究。
DOI：
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发表时间：
1994
影响因子：
3.2
通讯作者：
S. Vandenberg
中科院分区：
文献类型：
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作者：
T. Hirose;B. Scheithauer;M. Lopes;S. Vandenberg
为了评估包括胚胎发育不良性神经上皮肿瘤（DNT），特别是少突胶质细胞样细胞（OLC）的细胞的分化范围，用神经元和神经胶质标志物的谱免疫化学研究了14个DNT。八个肿瘤进行了超微结构研究。神经丝蛋白，III类β-微管蛋白和突触素制剂染色的一些OLC在两个，六个和一个病变，分别。此外，单个皮质结节内的许多OLC对III类β-微管蛋白有反应。绝大多数OLC呈S-100蛋白强阳性。胶质细胞酸性蛋白在两例中标记了相当数量的OLC，一个结节几乎完全由免疫反应阳性的星形胶质细胞组成。超微结构上，许多OLC类似于少突胶质细胞，表现出微管，突出的高尔基体和短细胞过程;细胞周层的细胞过程，少突胶质细胞的一个特征，注意到只有一个肿瘤。在两个案例中，OLC与星形胶质细胞的功能被视为包含少量的中间丝。在4例中，少数OLC类似于未成熟的神经元，缺乏致密核心颗粒或突触。这项研究证实了胶质神经元性质的DNT，病变的异质细胞组成，许多类似少突胶质细胞和少数显示早期星形胶质细胞和神经元分化。虽然它们与OLC的关系尚不清楚，但成熟神经元的存在和特殊分布仍然是病变的一个不可或缺的诊断特征。
To assess the range of differentiation of the cells comprising dysembryoplastic neuroepithelial tumor (DNT), particularly the oligodendrocyte-like cells (OLC), 14 DNT were immunochemically studied with a spectrum of neuronal and glial markers. Eight tumors were also studied ultrastructurally. Neurofilament protein, class III beta-tubulin, and synaptophysin preparations stained a few OLC in two, six and one lesion, respectively. In addition, many OLC within a single cortical nodule were reactive for class III beta-tubulin. The vast majority of OLC were strongly S-100 protein positive. Glial fibrillary acidic protein labeled a fair number of OLC in two cases and one nodule consisted almost entirely of immunoreactive astrocytes. Ultrastructurally, many OLC resembled oligodendrocytes in exhibiting microtubules, prominent Golgi and short cell processes; pericellular lamination of cell processes, a characteristic of oligodendroglia, was noted in only one tumor. In two cases, OLC with astrocytic features were seen to contain small numbers of intermediate filaments. In four cases, a few OLC resembled immature neurons with scant dense-core granules or synapses. This study confirms the glioneuronal nature of DNT, a lesion composed of heterogeneous cells, many resembling oligodendrocytes and a few showing early astrocytic and neuronal differentiation. Although their relation to OLC is unclear, the presence and peculiar distribution of mature neurons is nonetheless an integral diagnostic feature of the lesion.