Msc1 links dynamic Swi6/HP1 binding to cell fate determination.
Msc1 links dynamic Swi6/HP1 binding to cell fate determination.
复制标题
Msc1 将动态 Swi6/HP1 结合与细胞命运决定联系起来。
DOI：
10.1073/pnas.0811161106
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发表时间：
2009
影响因子：
11.1
通讯作者：
Volpe,ThomasA
中科院分区：
文献类型：
--
作者：
Lawrence,RichardJ;Volpe,ThomasA
真核生物的基因组可以被组织成异染色质或常染色质的不同结构域。在裂殖酵母裂殖酵母中，异染色质在沉默交配型区域的组装对于以交配型转换的形式决定细胞命运至关重要。在这里，我们报告说，泛素连接酶，MSC 1，是一个关键因素所需的适当的细胞命运决定在粟酒裂殖酵母。在没有Msc 1的情况下，Swi 6在异染色质灶的体内移动性受到损害，并且着丝粒异染色质变得超富集异染色质结合蛋白Swi 6/HP 1。然而，在交配型基因座，Swi 6招聘是有缺陷的MSC 1的情况下。因此，Msc 1将维持动态异染色质与适当的异染色质组装和细胞命运决定联系起来。这些发现对理解其他生物体的分化机制具有重要意义。
Eukaryotic genomes can be organized into distinct domains of heterochromatin or euchromatin. In the fission yeastSchizosaccharomyces pombe, assembly of heterochromatin at the silent mating-type region is critical for cell fate determination in the form of mating-type switching. Here, we report that the ubiquitin ligase, Msc1, is a critical factor required for proper cell fate determination inS. pombe. In the absence of Msc1, the in vivo mobility of Swi6 at heterochromatic foci is compromised, and centromere heterochromatin becomes hyperenriched with the heterochromatin binding protein Swi6/HP1. However, at the mating-type locus, Swi6 recruitment is defective in the absence of Msc1. Therefore, Msc1 links maintaining dynamic heterochromatin with proper heterochromatin assembly and cell fate determination. These findings have implications for understanding mechanisms of differentiation in other organisms.