Dynamic nature of cleavage bodies and their spatial relationship to DDX1 bodies, Cajal bodies, and gems
Dynamic nature of cleavage bodies and their spatial relationship to DDX1 bodies, Cajal bodies, and gems
复制标题
DOI：
10.1091/mbc.e05-08-0768
复制
发表时间：
2006-03-01
影响因子：
3.3
通讯作者：
Godbout, R
中科院分区：
文献类型：
--
作者：
Li, L;Roy, K;Godbout, R
DDX1小体、裂解小体、Cajal小体和宝石是核亚细胞器，含有参与RNA转录和/或加工的因子。虽然这四种核体都可以作为不同的实体存在，但它们往往是共域的或相互重叠的。为了更好地理解这四种核体之间的关系，我们研究了它们的空间分布作为细胞周期的函数。在这里，我们报道了DDX1体、CBs和宝石存在于整个间相，cpsf -100解理体主要存在于S相和G2相，而cstf -64解理体主要存在于S相。四种核体在S期相互结合，解理体与DDX1体共定位，解理体/DDX1体位于宝石和CBs附近。虽然RNA转录抑制剂对DDX1小体或切割小体没有影响，但DNA复制抑制剂导致含有cstf -64的切割小体丢失。肌动蛋白聚合抑制剂latrunculin B对核结构有显著影响，导致形成由CstF-64、CPSF-100、RNA和RNA聚合酶II组成的针状核针状体。我们的结果表明，解理体成分在本质上是高度动态的。
DDX1 bodies, cleavage bodies, Cajal bodies (CBs), and gems are nuclear suborganelles that contain factors involved in RNA transcription and/or processing. Although all four nuclear bodies can exist as distinct entities, they often colocalize or overlap with each other. To better understand the relationship between these four nuclear bodies, we examined their spatial distribution as a function of the cell cycle. Here, we report that whereas DDX1 bodies, CBs and gems are present throughout interphase, CPSF-100-containing cleavage bodies are predominantly found during S and G2 phases, whereas CstF-64-containing cleavage bodies are primarily observed during S phase. All four nuclear bodies associate with each other during S phase, with cleavage bodies colocalizing with DDX1 bodies, and cleavage bodies/DDX1 bodies residing adjacent to gems and CBs. Although inhibitors of RNA transcription had no effect on DDX1 bodies or cleavage bodies, inhibitors of DNA replication resulted in loss of CstF-64-containing cleavage bodies. A striking effect on nuclear structures was observed with latrunculin B, an inhibitor of actin polymerization, resulting in the formation of needlelike nuclear spicules made up of CstF-64, CPSF-100, RNA, and RNA polymerase II. Our results suggest that cleavage body components are highly dynamic in nature.