pbx is required for pole and eye regeneration in planarians
pbx is required for pole and eye regeneration in planarians
复制标题
DOI：
10.1242/dev.083741
复制
发表时间：
2013-02-15
期刊：
影响因子：
4.6
通讯作者：
Reddien, Peter W.
中科院分区：
文献类型：
--
作者：
Chen, Chun-Chieh G.;Wang, Irving E.;Reddien, Peter W.
Planarian再生涉及指定身体计划的区域化基因表达。在截肢后，涡虫能够再生新的前极和后极，以及沿着前后轴、背腹轴和内外轴极化的组织。Wnt和几个Hox基因在后极表达，而Wnt抑制基因、Fgf抑制基因和编码TALE家族同源结构域蛋白的pre在前极表达。我们发现，Smed-pbx（简称pbx），它编码的第二涡虫TALE家族同源结构域转录因子，是需要恢复这些基因在再生过程中的前极和后极的表达。此外，pbx（RNAi）动物在稳态期间逐渐失去极基因表达。相比之下，pbx是不需要的初始前-后极化反应的伤口，表明pbx是需要的伤口反应后，在再生和稳态组织周转过程中的两极的发展和维护。与pbx在极再生中的需求无关，pbx是眼前体形成所必需的，因此，眼再生和眼替换在稳态中是必需的。总之，这些数据表明，pbx促进身体轴杆的形成和眼睛再生祖细胞的形成。
Planarian regeneration involves regionalized gene expression that specifies the body plan. After amputation, planarians are capable of regenerating new anterior and posterior poles, as well as tissues polarized along the anterior-posterior, dorsal-ventral and medial-lateral axes. Wnt and several Hox genes are expressed at the posterior pole, whereas Wnt inhibitory genes, Fgf inhibitory genes, and prep, which encodes a TALE-family homeodomain protein, are expressed at the anterior pole. We found that Smed-pbx (pbx for short), which encodes a second planarian TALE-family homeodomain transcription factor, is required for restored expression of these genes at anterior and posterior poles during regeneration. Moreover, pbx(RNAi) animals gradually lose pole gene expression during homeostasis. By contrast, pbx was not required for initial anterior-posterior polarized responses to wounds, indicating that pbx is required after wound responses for development and maintenance of poles during regeneration and homeostatic tissue turnover. Independently of the requirement for pbx in pole regeneration, pbx is required for eye precursor formation and, consequently, eye regeneration and eye replacement in homeostasis. Together, these data indicate that pbx promotes pole formation of body axes and formation of regenerative progenitors for eyes.