Obtaining of CD34+ cells from healthy blood donors: development of a rapid and efficient procedure using leukoreduction filters
Obtaining of CD34+ cells from healthy blood donors: development of a rapid and efficient procedure using leukoreduction filters
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从健康献血者获取 CD34+ 细胞：使用白细胞去除过滤器开发快速有效的程序
DOI：
10.1111/j.1537-2995.2010.02683.x
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发表时间：
2010
期刊：
影响因子：
2.9
通讯作者：
V. Praloran
中科院分区：
文献类型：
--
作者：
Yann Peytour;Amélie V. Guitart;Arnaud Villacreces;J. Chevaleyre;F. Lacombe;Z. Ivanovic;V. Praloran
背景：人类 CD34+ 细胞对于研究人类造血的许多方面是必需的。它们在血液或骨髓中的频率较低，并且伦理原因限制了它们的大量获取。红细胞制备后，白细胞减少过滤器 (LRF) 被丢弃。健康供体血液中的 CD34+ 细胞浓度较低 (1 × 103‐4 × 103/mL)，但过滤 400 至 450 mL 血液后，在一个 LRF 中捕获的 CD34+ 细胞数量较高 (0.4 × 106‐1.6 × 106)。
BACKGROUND: Human CD34+ cells are mandatory to study many aspects of human hematopoiesis. Their low frequency in blood or marrow and ethical reasons limit their obtainment in large quantities. Leukoreduction filters (LRFs) are discarded after preparation of red blood cells. The CD34+ cell concentration in healthy donor blood is low (1 × 103‐4 × 103/mL), but their number trapped in one LRF after filtration of 400 to 450 mL of blood is high (0.4 × 106‐1.6 × 106).