A Bacillus-specific factor is needed to trigger the stress-activated phosphatase/kinase cascade of sigmaB induction.
A Bacillus-specific factor is needed to trigger the stress-activated phosphatase/kinase cascade of sigmaB induction.
复制标题
需要芽孢杆菌特异性因子来触发 sigmaB 诱导的应激激活磷酸酶/激酶级联。
DOI：
10.1006/bbrc.1999.0418
复制
发表时间：
1999
影响因子：
3.1
通讯作者：
Haldenwang,WG
中科院分区：
文献类型：
--
作者：
Scott,JM;Smirnova,N;Haldenwang,WG
枯草芽孢杆菌的一般应激调节因子由转录因子ςB控制。环境应激通过磷酸酶/激酶级联激活ςB，从而触发ςB从抗Sigma因子复合体中释放。为了确定磷酸酶/激酶级联的成员是否足以检测环境胁迫并激活ςB，我们表达了ςBand及其调节因子。Coli.InE.Coli，作为INB.枯草杆菌，完整的调控因子集合，使ςB沉默，同时允许ςB在级联最上游的负调控因子被删除的情况下激活。然而，监管机构无法激活ςBin，以应对乙醇处理或热休克。在其他实验中，GroEL和DNAK伴侣已知在控制压力ς因子方面具有重要作用。Coli，对ςB的活性不重要。研究结果表明，ςB的压力诱导需要新的B因子。枯草杆菌特有的。
The general stress regulon ofBacillus subtilisis controlled by the transcription factor ςB. Environmental stress activates ςBvia a phosphatase/kinase cascade that triggers ςB's release from an anti sigma factor complex. To determine if the members of the phosphatase/kinase cascade are sufficient to detect environmental stress and activate ςB, we expressed ςBand its regulators inE. coli.InE. coli,as inB. subtilis,the intact collection of regulators silenced ςB, while allowing ςBto be active if the cascade's most upstream negative regulator was deleted. The regulators could not, however, activate ςBin response to ethanol treatment or heat shock. In other experiments, the GroEL and DnaK chaperones, known to be important in controlling stress ς factors inE. coli,were found to be unimportant for ςBactivity inB. subtilis.The findings argue that stress induction of ςBrequires novel factors that areB. subtilisspecific.