Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China
Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China
复制标题
中国钩端螺旋体6个流行血清群快速分型O抗原基因簇特异性PCR方法的建立
DOI：
10.1186/1471-2180-10-67
复制
发表时间：
2010-03-03
期刊：
影响因子：
4.2
通讯作者：
Guo, Xiao-Kui
中科院分区：
文献类型：
--
作者：
Cai, Cheng-Song;Zhu, Yong-Zhang;Guo, Xiao-Kui
背景：钩端螺旋体是钩端螺旋体病的病原体。O-抗原是脂多糖的远端部分，脂多糖是革兰氏阴性菌外膜的关键组分，并赋予血清学特异性。钩端螺旋体病的流行病学和临床特征与血清学分类单位有关。结果：本研究对我国4个流行性钩端螺旋体血清群（犬群、秋群、流感伤寒群和七日群）的O抗原基因簇进行了测序，并对所有基因进行了计算机预测。通过添加已发表的黄疸出血群（Lai和Fiocruz L1-130株）和Sejroe（JB 197和L550株）两个血清群的序列，我们鉴定了中国6个流行血清群的6个O抗原特异性基因。利用这些基因建立的PCR检测方法对75株参考菌株和40株临床分离株进行了检测。结论：PCR检测方法可作为钩体6个血清型的快速分型方法。
Background: Leptospira is the causative agent of leptospirosis. The O-antigen is the distal part of the lipopolysaccharide, which is a key component of outer membrane of Gram-negative bacteria and confers serological specificity. The epidemiology and clinical characteristics of leptospirosis are relative to the serology based taxonomic unit. Identification of Leptospira strains by serotyping is laborious and has several drawbacks.Results: In this study, the O-antigen gene clusters of four epidemic Leptospira serogroups (serogroup Canicola, Autumnalis, Grippotyphosa and Hebdomadis) in China were sequenced and all genes were predicted in silico. Adding published sequences of two serogroups, Icterohaemorrhagiae (strain Lai and Fiocruz L1-130) and Sejroe (strain JB197 and L550), we identified six O-antigen-specific genes for six epidemic serogroups in China. PCR assays using these genes were developed and tested on 75 reference strains and 40 clinical isolates.Conclusion: The results show that the PCR-based assays can be reliable and alternative means for rapid typing of these six serogroups of Leptospira.