Drosophila chromosome condensation proteins topoisomerase II and Barren colocalize with Polycomb and maintain Fab-7 PRE silencing
Drosophila chromosome condensation proteins topoisomerase II and Barren colocalize with Polycomb and maintain Fab-7 PRE silencing
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DOI：
10.1016/s1097-2765(01)00161-7
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发表时间：
2001-01-01
期刊：
影响因子：
16
通讯作者：
Orlando, V
中科院分区：
文献类型：
--
作者：
Lupo, R;Breiling, A;Orlando, V
细胞记忆机制在染色质结构水平上控制细胞同一性的维持。我们已经调查了反之亦然；也就是说，负责维持染色体结构的功能是否在基因表达的表观遗传控制中发挥作用。我们发现拓扑异构酶II(TOPOII)和BARR(BARR)在体内与果蝇双胸复合体中的多梳群(PcG)靶序列相互作用，包括多梳状反应元件。此外，我们发现PcG蛋白多同源异型(PH)与TOPOII和BARR发生物理相互作用，并且BARR是Fab-7调节同源异型基因表达所必需的。相反，我们发现与ph突变相关的染色体分离缺陷。我们认为染色质凝聚蛋白参与了间期调节染色体结构域拓扑的机制，对维持基因表达是必不可少的。
Mechanisms of cellular memory control the maintenance of cellular identity at the level of chromatin structure. We have investigated whether the converse is true; namely, if functions responsible for maintenance of chromosome structure play a role in epigenetic control of gene expression. We show that Topoisomerase II (TOPOII) and Barren (BARR) interact in vivo with Polycomb group (PcG) target sequences in the bithorax complex of Drosophila, including Polycomb response elements. In addition, we find that the PcG protein Polyhomeotic (PH) interacts physically with TOPOII and BARR and that BARR is required for Fab-7-regulated homeotic gene expression. Conversely, we find defects in chromosome segregation associated with ph mutations. We propose that chromatin condensation proteins are involved in mechanisms acting in interphase that regulate chromosome domain topology and are essential for the maintenance of gene expression.