Stability of membrane potential in heart mitochondria: single mitochondrion imaging.
Stability of membrane potential in heart mitochondria: single mitochondrion imaging.
复制标题
DOI：
10.1016/j.bbrc.2006.03.233
复制
发表时间：
2006-06
影响因子：
3.1
通讯作者：
Yukiko Uechi;H. Yoshioka;D. Morikawa;Y. Ohta
中科院分区：
文献类型：
--
作者：
Yukiko Uechi;H. Yoshioka;D. Morikawa;Y. Ohta
线粒体膜电位（ΔΨm）在细胞活动中起重要作用。虽然ΔΨmof细胞内线粒体相对稳定，但最近对分离线粒体的实验表明，单个线粒体显示出ΔΨm的频繁波动。目前的研究是通过荧光显微镜观察ΔΨmof单个分离线粒体来研究稳定ΔΨmin细胞的因素。本研究发现：(1)质膜渗透细胞中的线粒体也会发生瞬时去极化；(2)从猪心脏中分离的几乎所有线粒体都表现出瞬时去极化，并且随着质子从基质向膜间空间的净流出而增强；(3)ATP和ADP通过多种机制显著抑制瞬时去极化。这些结果表明，抑制基质的急性碱化以及ATP和ADP的存在有助于ΔΨmin细胞的稳定。
Mitochondrial membrane potential (ΔΨm) plays an important role in cellular activity. Although ΔΨmof intracellular mitochondria are relatively stable, the recent experiments with isolated mitochondria demonstrate that individual mitochondria show frequent fluctuations of ΔΨm. The current study is performed to investigate the factors that stabilize ΔΨmin cells by observing ΔΨmof individual isolated mitochondria with fluorescence microscopy. Here, we report that (1) the transient depolarizations are also induced for mitochondria in plasma membrane permeabilized cells, (2) almost all mitochondria isolated from porcine hearts show the transient depolarizations that is enhanced with the net efflux of protons from the matrix to the intermembrane space, and (3) ATP and ADP significantly inhibit the transient depolarizations by plural mechanisms. These results suggest that the suppression of acute alkalinization of the matrix together with the presence of ATP and ADP contributes to the stabilization of ΔΨmin cells.