Neuroexcitatory actions of ciguatoxin on brain regions associated with thermoregulation.
Neuroexcitatory actions of ciguatoxin on brain regions associated with thermoregulation.
复制标题
DOI：
10.1097/00001756-199501000-00020
复制
发表时间：
1995
期刊：
影响因子：
1.7
通讯作者：
Y. Peng;T. B. Taylor;R. Finch;P. Moeller;J. Ramsdell
中科院分区：
文献类型：
--
作者：
Y. Peng;T. B. Taylor;R. Finch;P. Moeller;J. Ramsdell
本文研究了雪卡毒素(CTX)对小鼠腹腔注射后的作用。行政管理。CTX(0.5个小鼠单位)引起快速(10-20分钟)体温下降，并持续数小时。这与雪卡毒素对脑内c-fos基因的神经兴奋作用密切相关。我们通过c-fos翻译产物的免疫染色确定了CTX作用激活的小鼠大脑中的神经元通路，c-fos翻译产物是神经兴奋性的生物标记物。C-fos免疫反应阳性细胞主要分布在下丘脑，包括视前内侧核和视上核。在脑干的某些区域，包括蓝斑、臂旁背外侧核、最后区和孤束核，也有明显的免疫染色。这些研究表明，CTX对接受迷走神经传入的脑干区域以及与内脏和体温调节反应相关的上行通路具有神经兴奋作用。
We have investigated the action of ciguatoxin (CTX) in the mouse following i.p. administration. CTX (0.5 mouse units) induced a rapid (10-20 min) decrease in body temperature that persisted for several hours. This corresponded closely with a neuroexcitatory action of ciguatoxin on c-fos mRNA in the brain. We identified the neuronal pathways activated by CTX action in the mouse brain by immunostaining of c-fos translational product, a biomarker for neuroexcitability. c-fos-like immunoreactivity was prominent in the hypothalamus, including the medial preoptic and supraoptic nuclei. Immunostaining was also evident in certain regions of the brain stem, including the locus coeruleus, dorsolateral parabranchial nucleus, area postrema and the nucleus of the solitary tract. These studies indicate that CTX has neuroexcitatory actions on brain stem regions receiving vagal afferents and ascending pathways associated with visceral and thermoregulatory responses.