Levels of HTNV-specific CD8+ T lymphocytes in PBMC from the patients with hemorrhagic fever with renal syndrome
Levels of HTNV-specific CD8+ T lymphocytes in PBMC from the patients with hemorrhagic fever with renal syndrome
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DOI：
10.1007/s11739-011-0633-4
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发表时间：
2013-09-01
影响因子：
4.6
通讯作者：
Lv, Shemin
中科院分区：
文献类型：
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作者：
Xie, Ming;Dong, Yanying;Lv, Shemin
肾综合征出血热（HFRS）的发病机制尚未完全阐明。细胞介导的免疫似乎在 HFRS 的免疫发病机制中发挥着至关重要的作用。为了探讨汉坦（HTNV）特异性CD8（+）T淋巴细胞与HFRS免疫发病机制的关系，采用流式细胞术检测外周血单个核细胞中HTNV特异性CD8（+）T淋巴细胞分泌的干扰素γ（IFN-γ）水平。 HFRS患者HTNV特异性CD8+T淋巴细胞水平与HFRS的不同阶段相关。发热期则明显升高。 HFRS患者PBMC中HTNV特异性CD8(+)T淋巴细胞水平与血浆尿素氮、肌酐水平呈负相关。结果表明，HTNV 特异性 CD8(+) T 淋巴细胞水平与疾病阶段相关。因此，动态观察HFRS患者这些水平有助于判断HFRS疾病状态，阐明HFRS的免疫发病机制。
The pathogenesis of hemorrhagic fever with renal syndrome (HFRS) has not been fully clarified. Cell-mediated immunity appears to play a crucial role in the immune pathogenesis of HFRS. To explore the relationship between Hantaan (HTNV)-specific CD8(+) T lymphocytes and the immune pathogenesis of HFRS, the levels of interferon gamma (IFN-gamma) secreted by HTNV-specific CD8(+) T lymphocytes were detected by flow cytometry in peripheral blood mononuclear cells. Levels of HTNV-specific CD8(+) T lymphocytes in patients with HFRS were associated with different phases of HFRS. In fever phase, it was significantly elevated. The levels of HTNV-specific CD8(+) T lymphocytes in PBMC of patients with HFRS were negatively correlated with the levels of blood urea nitrogen and creatinine in plasma. The results show that the HTNV-specific CD8(+) T lymphocyte levels correlate with disease phases. Therefore, dynamic observation of these levels in patients with HFRS can help to judge the status of HFRS disease and to clarify the immune pathogenesis of HFRS.