Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa
Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa
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DOI：
10.1515/pjvs-2017-0011
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发表时间：
2017-01-01
影响因子：
0.8
通讯作者：
Kordan, W.
中科院分区：
文献类型：
--
作者：
Dziekonska, A.;Swiader, K.;Kordan, W.
本研究的目的是探讨精液中富含精子的部分（F1）和F1后的部分（F2）对猪精子液态保存质量的影响。从三头波兰长白猪中收集精液。用BTS短期稀释剂和Safe-Cell Plus（SCP）长期稀释剂稀释每种精液级分，并在17 ℃下储存7天（D1-D 7）。分析包括精子活力参数、正常顶脊（NAR）顶体和质膜完整性（PMI）。在稀释级分之前，
The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17 degrees C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p