Altered prostaglandin synthesis by glomeruli from rats with unilateral ureteral ligation.
Altered prostaglandin synthesis by glomeruli from rats with unilateral ureteral ligation.
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单侧输尿管结扎改变大鼠肾小球的前列腺素合成。
DOI：
10.1152/ajprenal.1981.241.3.f289
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Schlondorff,D
中科院分区：
文献类型：
--
作者：
Folkert,VW;Schlondorff,D
有证据表明，前列腺素对单侧输尿管梗阻的肾脏血流动力学有调节作用。我们检测了单侧输尿管结扎24小时和72小时的大鼠肾小球前列腺素的合成。在用[14C]花生四烯酸肾小球预先标记脂质池的过程中，来自结扎肾脏的~(14)C标记比来自对侧或正常对照肾脏的~(14)C标记更少。然而，在结扎的肾脏中，[14C]花生四烯酸掺入肾小球磷脂的比例较高。预先标记的肾小球与血管紧张素II或缓激肽孵育显著增加结扎侧标记前列腺素的释放和[14C]花生四烯酸的周转。放射免疫测定显示，结扎24小时后，两侧肾小球产生等量的前列腺素F2α、6-酮-前列腺素F1α、血栓素B2和前列腺素E_2。72 h后，结扎肾小球产生的PGF2α、6-keto-PGF1α和TXB2均高于对侧肾小球。相反，对侧肾小球PGE2合成较高。血管紧张素II和缓激肽对可放射免疫测定的前列腺素均无明显影响。我们得出结论，单侧输尿管结扎与肾小球前列腺素合成和磷脂代谢的特异性改变有关，这些都是肾小球固有的。
There is evidence that prostaglandins modulate renal hemodynamics in unilateral ureteral obstruction. We examined prostaglandin synthesis by glomeruli from rats with 24- and 72-h unilateral ureteral ligation. During prelabeling of the lipid pools with [14C]arachidonate glomeruli from the ligated kidney incorporated less 14C label than those from contralateral or normal control kidneys. However, the incorporation of [14C]arachidonate into phospholipids of glomeruli was proportionately higher in the ligated kidney. Incubation of the prelabeled glomeruli with either angiotensin II or bradykinin significantly increased release of labeled prostaglandins and turnover of [14C]arachidonate on the ligated side. Radioimmunoassay determinations showed that after 24-h ligation, glomeruli from both sides produced comparable amounts of PGF2 alpha, 6-keto-PGF1 alpha, TXB2, and PGE2. After 72 h, glomeruli from the ligated kidney produced more PGF2 alpha, 6-keto-PGF1 alpha, and TXB2 than the contralateral glomeruli. In contrast, PGE2 synthesis was higher in contralateral glomeruli. Neither angiotensin II nor bradykinin had a measurable effect on radioimmunoassayable prostaglandins. We conclude that unilateral ureteral ligation is associated with specific alterations of glomerular prostaglandin synthesis and phospholipid metabolism that are intrinsic to the glomerulus.