Prevascularization of cardiac patch on the omentum improves its therapeutic outcome
Prevascularization of cardiac patch on the omentum improves its therapeutic outcome
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DOI：
10.1073/pnas.0812242106
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发表时间：
2009-09-01
影响因子：
11.1
通讯作者：
Cohen, Smadar
中科院分区：
文献类型：
--
作者：
Dvir, Tal;Kedem, Alon;Cohen, Smadar
心脏贴片生物工程的最新进展为心肌梗死（MI）后心肌的再生提供了一种新的治疗方式。我们在这里提出了一种通过异位移植到大网膜上来工程化具有成熟脉管系统的心脏补片的策略。该贴片的构建方法是将新生儿心肌细胞与促存活因子和血管生成因子的混合物接种到能够因子结合和持续释放的藻酸盐支架中。培养48小时后，贴片在大网膜上血管化7天，然后在MI诱导7天后移出并移植到梗塞大鼠心脏上。 28 天后进行评估时，血管化心脏补片显示出与宿主心肌的结构和电整合。此外，血管化补片会引起更厚的疤痕，防止心室进一步扩张和心室功能障碍。因此，我们的研究提供了证据，证明将预血管化的心脏补片移植到梗塞处可以改善 MI 后的心脏功能。
The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting pre-vascularized cardiac patch into infarct can improve cardiac function after MI.