An ATMND/SGI based label-free and fluorescence ratiometric aptasensor for rapid and highly sensitive detection of cocaine in biofluids
An ATMND/SGI based label-free and fluorescence ratiometric aptasensor for rapid and highly sensitive detection of cocaine in biofluids
复制标题
基于 ATMND/SGI 的无标记荧光比率适体传感器，用于快速、高灵敏地检测生物体液中的可卡因
DOI：
10.1016/j.talanta.2016.08.039
复制
发表时间：
2016-12-01
期刊：
影响因子：
6.1
通讯作者：
Meng, Changgong
中科院分区：
文献类型：
--
作者：
Wang, Jiamian;Song, Jie;Meng, Changgong
研制了一种无标记比例荧光配体传感器，用于快速、灵敏地检测复杂生物流体中的可卡因。该荧光适体传感器由未标记的GC-38可卡因适体作为基本传感单元和2-氨基-5,6,7-三甲基-1,8-萘啶（ATMND）和SYBR Green I （SGI）两个荧光基团组成，分别作为信号报告蛋白和内置参考蛋白。检测原理是基于可卡因特异性介导的ATMND位移反应以及相应的ATMND与SGI荧光比值的变化。由于非标记适配体的高亲和力，源自比例法的良好精度，以及荧光团的良好荧光量子产率，该适配体传感器在可卡因检测方面表现出良好的分析性能。在最佳条件下，该传感器线性范围为0.10 ~ 10 μ M，检测下限为56 nM，快速响应时间为20 s。检测下限与大多数具有信号放大策略的荧光适体传感器相当，远低于所有未扩增的可卡因适体传感器。对包括尿液、唾液和血清在内的一系列复杂生物流体的实际样品分析也表明，该适体传感器具有良好的精度、稳定性和高灵敏度，可能在复杂生物流体中的可卡因即时筛查方面具有很大的潜力。(C) 2016 Elsevier B.V.版权所有
A label-free ratiometric fluorescence aptasensor has been developed for the rapid and sensitive detection of cocaine in complex biofluids. The fluorescent aptasensor is composed of a non-labeled GC-38 cocaine aptamer which serves as a basic sensing unit and two fluorophores, 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) and SYBR Green I (SGI) which serves as a signal reporter and a build-in reference, respectively. The detection principle is based on a specific cocaine mediated ATMND displacement reaction and the corresponding change in the fluorescence ratio of ATMND to SGI. Due to the high affinity of the non-labeled aptamer, the good precision originated from the ratiometric method, and the good fluorescence quantum yield of the fluorophore, the aptasensor shows good analytical performance with respect to cocaine detection. Under optimal conditions, the aptasensor shows a linear range of 0.10-10 mu M and a low limit of detection of 56 nM, with a fast response of 20 s. The low limit of detection is comparable to most of the fluorescent aptasensors with signal amplification strategies and much lower than all of the unamplified cocaine aptasensors. Practical sample analysis in a series of complex biofluids, including urine, saliva and serum, also indicates the good precision, stability, and high sensitivity of the aptasensor, which may have great potential for the point-of-care screening of cocaine in complex biofluids. (C) 2016 Elsevier B.V. All rights reserved.