Anti-Inflammatory Activity of Chemical Constituents Isolated from the Willow Bracket Medicinal Mushroom Phellinus igniarius (Agaricomycetes)
Anti-Inflammatory Activity of Chemical Constituents Isolated from the Willow Bracket Medicinal Mushroom Phellinus igniarius (Agaricomycetes)
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DOI：
10.1615/intjmedmushrooms.2018025536
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发表时间：
2018-01-01
影响因子：
1.2
通讯作者：
Li, Gao
中科院分区：
文献类型：
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作者：
Jiang, Zhe;Jin, Mei;Li, Gao
核因子-κ B（NF-κ B）在免疫应答和炎症反应中具有重要作用。桑黄具有多种药理作用。本研究从黄药子实体中分离得到19个化学成分（1-19）。根据核磁共振波谱数据并与文献比较，对它们的结构进行了解析。从该种植物中共分离得到3个化合物（2、7、11），为首次报道。其中2个化合物（4和15）为首次报道，9个化合物（1、3、5、8-10和12-14）为首次报道。这些化合物的NF-κ B荧光素酶报告基因测定在肿瘤坏死因子-α诱导的HeLa细胞中进行-再次，据我们所知，这是第一次。其中，化合物5和7在100 μ mol/L时在HeLa细胞中显示出对NF-κ B的中度抑制，倍数值分别为0.48 +/- 0.02和0.55 +/- 0.09。这些结果表明，一些成分，从火棘可以开发成药物，用于临床应用。
Nuclear factor-kappa B (NF-kappa B) has an important role in immune response and inflammation. Phellinus igniarius (known as Sang huang in traditional Chinese medicine) has various pharmacologic effects. In this study, 19 chemical constituents (1-19) were isolated from the fruiting bodies of Ph. igniarius. Their structures were elucidated based on data from nuclear magnetic resonance spectroscopy and comparison with the literature. Overall, 3 compounds (2, 7, and 11) from this species were reported, to our knowledge for the first time. Two compounds (4 and 15) from the genus Phellinus and 9 compounds (1, 3, 5, 8-10, and 12-14) from the Hymenochaetaceae family were also reported for the first time. An NF-kappa B luciferase reporter assay of these compounds was carried out in tumor necrosis factor-alpha-induced HeLa cells-again, to our knowledge for the first time. Among them, compounds 5 and 7 showed moderate inhibition of NF-kappa B, with fold values of 0.48 +/- 0.02 and 0.55 +/- 0.09, respectively, in HeLa cells at 100 mu mol/L. These results suggest that some of the ingredients from Ph. igniarius could be developed into antiinflammatory agents for use in clinical applications.