EVIDENCE FOR COMMON ANCESTRY OF A CHESTNUT BLIGHT HYPOVIRULENCE-ASSOCIATED DOUBLE-STRANDED-RNA AND A GROUP OF POSITIVE-STRAND RNA PLANT-VIRUSES
EVIDENCE FOR COMMON ANCESTRY OF A CHESTNUT BLIGHT HYPOVIRULENCE-ASSOCIATED DOUBLE-STRANDED-RNA AND A GROUP OF POSITIVE-STRAND RNA PLANT-VIRUSES
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DOI：
10.1073/pnas.88.23.10647
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发表时间：
1991-12-01
影响因子：
11.1
通讯作者：
CARRINGTON, JC
中科院分区：
文献类型：
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作者：
KOONIN, EV;CHOI, GH;CARRINGTON, JC
对大病毒样双链RNA（L-dsRNA）中存在的两个开放阅读框编码的推定多肽产物进行计算机辅助分析，与板栗枯病真菌Cryphonectria parasitica的低毒力相关，揭示了五个不同的结构域，与之前描述的植物马铃薯Y病毒编码多蛋白内的保守结构域具有显着的序列相似性。这些包括假定的RNA依赖性RNA聚合酶、RNA解旋酶、与马铃薯Y病毒属辅助成分蛋白酶相关的两种木瓜蛋白酶样半胱氨酸蛋白酶，以及与马铃薯Y病毒属辅助成分蛋白的N末端部分相似的功能未知的富含半胱氨酸的结构域。使用三种独立的算法，从L-dsRNA（正链RNA病毒的一个子集）和双链RNA病毒的聚合酶结构域的比对中衍生出系统发育树，表明低毒力相关的dsRNA和马铃薯Y病毒基因组具有共同的祖先。然而，对各个病毒编码的多蛋白内保守结构域的组织的比较表明，所提出的后续进化涉及广泛的基因组重排。
Computer-assisted analysis of the putative polypeptide products encoded by the two open reading frames present in a large virus-like double-stranded RNA, L-dsRNA, associated with hypovirulence of the chestnut blight fungus, Cryphonectria parasitica, revealed five distinct domains with significant sequence similarity to previously described conserved domains within plant potyvirus-encoded polyproteins. These included the putative RNA-dependent RNA polymerase, RNA helicase, two papain-like cysteine proteases related to the potyvirus helper-component protease, and a cysteine-rich domain of unknown function similar to the N-terminal portion of the potyvirus helper-component protein. Phylogenetic trees derived from the alignment of the polymerase domains of L-dsRNA, a subset of positive-stranded RNA viruses, and double-stranded RNA viruses, using three independent algorithms, suggested that the hypovirulence-associated dsRNA and potyvirus genomes share a common ancestry. However, comparison of the organization of the conserved domains within the encoded polyproteins of the respective viruses indicated that the proposed subsequent evolution involved extensive genome rearrangement.