Bone marrow tissue and proliferation markers: Results and general problems
Bone marrow tissue and proliferation markers: Results and general problems
复制标题
骨髓组织和增殖标志物：结果和一般问题
作者：
J. Thiele;R. Fischer
一般来说，细胞增殖的评估可以通过多种方法来实现，其中包括有丝分裂计数、氚化胸苷标记指数、溴脱氧尿苷掺入、流式细胞术、银染核仁组织区（AgNOR）和免疫组织化学。为了评估经过常规处理的人体组织的增殖活性，有丝分裂计数是一种相对简单的测量增殖的方法。然而，有丝分裂数与增殖之间的关系并不像预期的那么密切，因为不同细胞周期阶段的持续时间存在很大程度的差异，并且众所周知，区分有丝分裂中的细胞与固缩细胞核或其他人工制品存在困难（Baak 1990）。除了 AgNOR 之外，最近还鉴定了几种核蛋白，它们被认为是循环或非静止细胞的特征（Brown 和 Garter 1990；Hall 和 Levinson 1990；Quinn 和 Wright 1990；Linden 等人 1992；Yu 等人 1992a；Kreipe 等人 1993a；Schwarting 1993）。在过去几年中，已经产生了许多针对这些核蛋白的单克隆抗体，目的是确定经过福尔马林固定和石蜡包埋的组织材料的增殖潜力，更准确地说（Galand 和 Degraef 1989；Van Dierendonck 等人 1991；Cattoretti 等人 1992；Linden 等人 1992；Sawhney 和 Hall 1992 年；麦考密克等人，1993 年）。使用文件中的组织块来估计各种恶性肿瘤中的生长分数（处于细胞周期的 GI-、S-、G2-、M 期的细胞）的可能性已被证明是组织学的一种非常有用的辅助手段。在这种情况下，涉及指示增殖能力的标记物的免疫组织学方法似乎
Generally, assessment of cell proliferation may be achieved by a variety of methods amongst which are mitotic count, tritiated thymidine labelling index, bromo-deoxyuridine incorporation, flow cytometry, silverstained nucleolar organizer regions (AgNORs) and immunohistochemistry. For an evaluation of proliferative activity in human tissues which have been processed routinely, the mitotic count is a relatively simple way to measure proliferation. However, the relationships between numbers of mitotic figures and proliferation are not as close as might be expected, because of the great degree of variation in the duration of the different cellcycle phases and the well-known difficulties in discriminating cells in mitosis from pyknotic nuclei or other artefacts (Baak 1990). In addition to AgNORs, several nuclear proteins have been recently identified that are believed to characterize cycling or non-quiescent cells (Brown and Garter 1990; Hall and Levinson 1990; Quinn and Wright 1990; Linden et al. 1992; Yu et al. 1992a; Kreipe etal. 1993a; Schwarting 1993). In the last few years a number of monoclonal antibodies directed against these nuclear proteins have been raised with the aim of determining the proliferative potential of tissue material which has undergone formalin fixation and paraffin-wax embedding, more precisely (Galand and Degraef 1989; Van Dierendonck et al. 1991 ; Cattoretti et al. 1992; Linden et al. 1992; Sawhney and Hall 1992; Kreipe et al. 1993 a; McCormick et al. 1993 a). The possibility of using tissue blocks from files for an estimation of the growth fraction (cells in GI-, S-, G2-, Mphase of cell-cycle) in various malignancies has already been proven to be a very informative adjunct to histology. In this context immunohistological methods involving markers that indicate proliferative capacity seem to
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DOI：
10.1172/jci109989
发表时间：
1980
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Adamson,JW;Singer,JW;Catalano,P;Murphy,S;Lin,N;Steinmann,L;Ernst,C;Fialkow,PJ
通讯作者：
Fialkow,PJ
人类成纤维细胞中的 DNA 修复合成需要 DNA 聚合酶 delta。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nishida,C;Reinhard,P;Linn,S
通讯作者：
Linn,S
白血病的细胞动力学。
影响因子：
3.6
作者：
Andreeff,M
通讯作者：
Andreeff,M
评估膀胱移行细胞癌细胞增殖的五种组织病理学方法的比较。
影响因子：
3.3
作者：
Cohen,MB;Waldman,FM;Carroll,PR;Kerschmann,R;Chew,K;Mayall,BH
通讯作者：
Mayall,BH
Ph慢性粒细胞白血病白血病和正常造血细胞的生长特征及强化治疗效果。
影响因子：
20.3
作者：
Goto,T;Nishikori,M;Arlin,Z;Gee,T;Kempin,S;Burchenal,J;Strife,A;Wisniewski,D;Lambek,C;Little,C;Jhanwar,S;Chaganti,R;Clarkson,B
通讯作者：
Clarkson,B