MAPPING OF THE MOUSE X-CHROMOSOME USING RANDOM GENOMIC PROBES AND AN INTERSPECIFIC MOUSE CROSS
MAPPING OF THE MOUSE X-CHROMOSOME USING RANDOM GENOMIC PROBES AND AN INTERSPECIFIC MOUSE CROSS
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DOI：
10.1002/j.1460-2075.1985.tb04137.x
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发表时间：
1985-12-30
期刊：
影响因子：
11.4
通讯作者：
AVNER, PR
中科院分区：
文献类型：
--
作者：
AMAR, LC;ARNAUD, D;AVNER, PR
已在大肠杆菌中构建了两个富集鼠X染色体材料的文库。载体NM 1149从流式分选染色体。纯化了一系列独特的基因组序列DNA，并通过与一组体细胞杂交系杂交来表征其X染色体特异性。在前五个这样的X染色体特异性探针的特征，所有检测到的限制性片段长度多态性（RFLP）之间的近交系小鼠实验室品系，如C57 BL/6和BALB/c和SPE/Pas小鼠品系建立从野生小鼠spretus小鼠，当它们的DNA与限制性内切酶TaqI消化。利用这些RFLP的优势，所有五个探针已被定位在X染色体上使用种间回交B6 CBARI和SPE/Pas小鼠品系分离的X染色体标记次黄嘌呤磷酸核糖转移酶（Hprt）和Tabby（Ta）。三个探针映射到着丝粒和Hprt之间的区域，和两个远端Ta。由于这种X特异性序列探针检测M. spretus和M.高频率的小家鼠DNA，一个很大的面板，以及定位探针应该很快可用于与X染色体相关的生物学问题的研究，可以最好地使用鼠类物种接近。
Two libraries enriched in murine X chromosome material have been constructed in the .lambda. vector NM 1149 from flow-sorted chromosomes. Inserts of unique genomic sequence DNA were purified and their X chromosome specificity characterised by hybridisation to a panel of somatic cell hybrid lines. Of the first five such X chromosome-specific probes characterised, all detected restriction fragment length polymorphisms (RFLPs) between inbred mouse laboratory strains such as C57BL/6 and BALB/c and the SPE/Pas mouse strain established from a wild Mus spretus mouse, when their DNAs are digested with the restriction enzyme TaqI. Taking advantage of these RFLPs, all five probes have been localised on the X chromosome using an interspecific backcross between the B6CBARI and SPE/Pas mouse strains segregating the X chromosome markers hypoxanthine phosphoribosyl transferase (Hprt) and Tabby (Ta). Three of the probes map to the region between the centromere and Hprt, and two distal to Ta. Since such X-specific sequence probes detect RFLPs between M. spretus and M. musculus domesticus DNAs with high frequency, a large panel of well localised probes should soon be available for studies of biological problems associated with the X chromosome which can best be approached using the murine species.