Melanogenesis inhibitors from the desert plant Anastatica hierochuntica in B16 melanoma cells
Melanogenesis inhibitors from the desert plant Anastatica hierochuntica in B16 melanoma cells
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DOI：
10.1016/j.bmc.2010.01.046
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发表时间：
2010-03-15
影响因子：
3.5
通讯作者：
Yoshikawa, Masayuki
中科院分区：
文献类型：
--
作者：
Nakashima, Souichi;Matsuda, Hisashi;Yoshikawa, Masayuki
从埃及草药Anastatica hierochuntica整株植物中提取的甲醇提取物被发现可以抑制茶碱刺激的小鼠B16黑色素瘤4A5细胞中的黑色素生成。分离得到的化合物有：水飞蓟素A、水飞蓟素A、异水飞蓟素A、异水飞蓟素A、叶黄素、木犀草素、山奈酚、槲皮素、希罗秦素A和B、(2R,3S)-2,3-二氢-2-（3,4-二甲氧基苯基）-3-羟甲基-5-（2-甲酰基乙烯基）-7-羟基苯并呋喃、(+)-脱氢二甲氧基醇、(+)-balanophonin、1-（4-羟基-3-甲氧基苯基）-2-{4-[(E)-3-羟基-1-丙烯基]-2-甲氧基苯氧基}-1,3-丙二醇、3,4-二羟基苯甲醛显著抑制黑素形成，IC50值为6.1 ~ 32 μ m。至于水飞蓟宾和异水飞蓟宾的作用机制，酪氨酸酶活性的抑制可能是重要的。此外，异水飞蓟宾A和B抑制TRP-2的mRNA表达，但水飞蓟宾A和B在10和/或30 μ M时相反地增强酪氨酸酶和TRP-1和-2的mRNA表达，并且抑制细胞外信号调节激酶（ERK1/2）的磷酸化参与了mRNA表达的增强，至少部分类似于PD98059。(C) 2010 Elsevier Ltd.版权所有。
The methanolic extract from the whole plants of Anastatica hierochuntica, an Egyptian herbal medicine, was found to inhibit melanogenesis in theophylline-stimulated murine B16 melanoma 4A5 cells. Among the constituents isolated, anastatin A, silybin A, isosilybins A and B, eriodictyol, luteolin, kaempferol, quercetin, hierochins A and B, (2R,3S)-2,3-dihydro-2-(3,4-dimethoxyphenyl)-3-hydroxymethyl-5-(2-formylvinyl)-7-hydroxybenzofuran, (+)-dehydrodiconiferyl alcohol, (+)-balanophonin, 1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-hydroxy-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol, and 3,4-dihydroxybenzaldehyde substantially inhibited melanogenesis with IC50 values of 6.1-32 mu M. With regard to the mechanism of action of silybins and isosilybins, the inhibition of tyrosinase activity suggested to be important. In addition, isosilybins A and B inhibited the mRNA expression of TRP-2, but silybins A and B oppositely enhanced the mRNA expression of tyrosinase and TRP-1 and -2 at 10 and/or 30 mu M, and the inhibition of phosphorylation of extracellular signal-regulated kinases (ERK1/2) is involved in the enhanced expression of mRNA, at least in part, similar to that of PD98059. (C) 2010 Elsevier Ltd. All rights reserved.