Microencapsulation of Bacillus subtilis B99-2 and its biocontrol efficiency against Rhizoctonia solani in tomato
Microencapsulation of Bacillus subtilis B99-2 and its biocontrol efficiency against Rhizoctonia solani in tomato
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DOI：
10.1016/j.biocontrol.2015.05.013
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发表时间：
2015-11-01
影响因子：
4.2
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Ma, Xin;Wang, Xiaobing;Wang, Wei
生物防治是保护植物免受疾病侵染的一种很有前途的方法。枯草芽孢杆菌在农业上被广泛应用于促进植物生长和生物防治。然而，它们的保质期较短，限制了生物农药的应用。本研究旨在以麦芽糊精和阿拉伯胶为壁材，建立枯草杆菌B99-2微胶囊化工艺，以确定喷雾干燥微胶囊化的最佳条件，评价微胶囊的储存稳定性，并在田间条件下评价其对番茄纹枯病菌的生防效果。我们用不同浓度的壁材喷雾干燥对芽孢杆菌菌体进行微胶囊化。麦芽糊精是一种有效的壁材，特别是在浓度高于80%时，而阿拉伯胶不影响细菌存活率。当喷雾干燥温度为145℃、进料流量为550mLh-1、喷雾压力为0.15 Mpa时，枯草杆菌的平均存活率可达90%以上。枯草杆菌微胶囊保存540d后存活率为87.53%，比可湿性粉剂的保质期长。当用量为300ghm-2时，对番茄纹枯病菌的生防效果达79.91%，在田间条件下对番茄纹枯病菌的防治效果优于福美双可湿性粉剂。这些特性表明枯草杆菌微胶囊有可能成为一种成功的生防产品。(C)2015 Elsevier Inc.保留所有权利。
Biological control is a promising approach to protecting plants from disease. Bacillus subtilis has been widely used in agriculture for promoting plant growth and biocontrol. However, their short shelf life limits the application of biological pesticides. The objectives of this study were to develop a microencapsulation procedure of B. subtilis B99-2 using maltodextrin and gum arabic as wall materials to determine the optimum conditions of spray-drying in microencapsulation, evaluate storage stability of microcapsules, and assess their biocontrol efficiency against Rhizoctonia solani in tomato under field conditions. We microencapsulated the Bacillus thallus by spray-drying with various concentrations of the wall material. Maltodextrin was found to be an efficient wall material, especially at concentrations higher than 80%, while gum arabic did not affect the bacterial survival rate. The mean survival rate of B. subtilis was more than 90%, when spray drying was performed at 145 degrees C, with a feed flow rate of 550 mL h(-1), and a spray pressure of 0.15 MPa. B. subtilis microcapsule survival rate was 87.53% after 540 d of storage, which was a longer shelf life than that of wettable powders. Moreover, its biocontrol efficacy reached 79.91% when a dosage of 300 g hm(-2) was used, the microcapsule showed higher control efficacy than Thiram wettable powder against R. solani in tomato under field conditions. All these characteristics indicated that B. subtilis microcapsules have the potential to become a successful biocontrol product. (C) 2015 Elsevier Inc. All rights reserved.