SWR1 Chromatin-Remodeling Complex Subunits and H2A.Z Have Non-overlapping Functions in Immunity and Gene Regulation in Arabidopsis.
SWR1 Chromatin-Remodeling Complex Subunits and H2A.Z Have Non-overlapping Functions in Immunity and Gene Regulation in Arabidopsis.
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DOI：
10.1016/j.molp.2016.04.003
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发表时间：
2016-07-06
期刊：
影响因子：
27.5
通讯作者：
Kumar SV
中科院分区：
文献类型：
--
作者：
Berriri S;Gangappa SN;Kumar SV
组蛋白变体H2A的结合。Z通过SWR1染色质重塑复合体进入核小体是真核生物基因调控的关键步骤。在拟南芥中，SWR1c和H2AZ已被证明可以控制发育和环境反应背后的基因表达。虽然它们与防御有关，但复杂亚基和H2A的具体作用。Z在免疫中的作用还没有被很好地理解。在这项研究中，我们分析了SWR1c亚基，光周期独立早期开花1 (PIE1)，肌动蛋白相关蛋白6 (ARP6)， SWR1复合物6 （SWC6）以及H2A的作用。Z代表防御和基因调控。我们发现SWR1c成分在对不同病原体的抗性中发挥不同的作用。PIE1和SWC6功能的丧失以及H2A的耗竭。Z导致基础抗性降低，而ARP6功能的丧失导致抗性增强。我们发现PIE1和SWC6的突变导致效应触发免疫受损。SWR1c成分和H2A突变。Z也导致茉莉酸/乙烯介导的免疫受损。全基因组表达分析同样揭示了H2A的不同作用。Z和SWR1c组分在基因调控中的作用，并提示PIE1在防御信号通路间串扰调控中的潜在作用。我们的数据表明，虽然它们是同一个复合体的一部分，但拟南芥SWR1c成分在植物免疫和基因调控中可能具有非冗余功能。SWR1染色质重塑复合体亚基表现出功能特化和不重叠的作用。而PIE1、SWC6和H2A。Z是抗性的正调节因子，ARP6是抗性的负调节因子。结果表明，拟南芥SWR1c成分在植物免疫和基因调控中可能具有非冗余功能。
Incorporation of the histone variant H2A.Z into nucleosomes by the SWR1 chromatin remodeling complex is a critical step in eukaryotic gene regulation. In Arabidopsis, SWR1c and H2A.Z have been shown to control gene expression underlying development and environmental responses. Although they have been implicated in defense, the specific roles of the complex subunits and H2A.Z in immunity are not well understood. In this study, we analyzed the roles of the SWR1c subunits, PHOTOPERIOD-INDEPENDENT EARLY FLOWERING1 (PIE1), ACTIN-RELATED PROTEIN6 (ARP6), and SWR1 COMPLEX 6 (SWC6), as well as H2A.Z, in defense and gene regulation. We found that SWR1c components play different roles in resistance to different pathogens. Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance, while loss of ARP6 fucntion resulted in enhanced resistance. We found that mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity. Mutation in SWR1c components and H2A.Z also resulted in compromised jasmonic acid/ethylene-mediated immunity. Genome-wide expression analyses similarly reveal distinct roles for H2A.Z and SWR1c components in gene regulation, and suggest a potential role for PIE1 in the regulation of the cross talk between defense signaling pathways. Our data show that although they are part of the same complex, Arabidopsis SWR1c components could have non-redundant functions in plant immunity and gene regulation. SWR1 chromatin remodeling complex subunits show functional specialization and have non-overlapping roles. While PIE1, SWC6 and H2A.Z are positive regulator of resistance to pathogens, ARP6 acts as a negative regulator. Our results show that Arabidopsis SWR1c components could have non-redundant functions in plant immunity and gene regulation.