Cortical Dependence of Whisker Responses in Posterior Medial Thalamus In Vivo.
Cortical Dependence of Whisker Responses in Posterior Medial Thalamus In Vivo.
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晶须反应在体内内侧丘脑中的皮质依赖性。
DOI：
10.1093/cercor/bhw144
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Groh A
中科院分区：
文献类型：
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作者：
Mease RA;Sumser A;Sakmann B;Groh A
麻醉啮齿动物皮质层5B （L5B）厚簇锥体神经元对须刺激有可靠的反应。这些细胞驱动皮质丘脑通路，唤起丘脑后内侧核（POm）的尖峰。虽然POm的一个子集已被证明整合皮层L5B和平行神经信号，但大多数POm神经元被认为只接收来自L5B的驱动输入。在这里，我们通过研究POm中须诱发反应的起源，特别是L5B-POm通路的贡献，来验证这种可能性。我们比较了氨基甲酸乙酯麻醉小鼠的L5B峰和POm峰以及对须偏转的阈下反应。我们发现记录的POm神经元子集显示早期（<50 ms）尖峰反应和早期大epsp。在这些神经元中，早期的大EPSPs符合L5B输入标准，被皮质抑制阻断，并且还与自发的Up状态耦合的大EPSPs相互作用。这一结果支持POm细分的观点，其中一个主要通过L5B神经元接收晶须信号。
Cortical layer 5B (L5B) thick-tufted pyramidal neurons have reliable responses to whisker stimulation in anesthetized rodents. These cells drive a corticothalamic pathway that evokes spikes in thalamic posterior medial nucleus (POm). While a subset of POm has been shown to integrate both cortical L5B and paralemniscal signals, the majority of POm neurons are suggested to receive driving input from L5B only. Here, we test this possibility by investigating the origin of whisker-evoked responses in POm and specifically the contribution of the L5B-POm pathway. We compare L5B spiking with POm spiking and subthreshold responses to whisker deflections in urethane anesthetized mice. We find that a subset of recorded POm neurons shows early (<50 ms) spike responses and early large EPSPs. In these neurons, the early large EPSPs matched L5B input criteria, were blocked by cortical inhibition, and also interacted with spontaneous Up state coupled large EPSPs. This result supports the view of POm subdivisions, one of which receives whisker signals predominantly via L5B neurons.