Maternal obesity is not associated with placental lipid accumulation in women with high omega-3 fatty acid levels
Maternal obesity is not associated with placental lipid accumulation in women with high omega-3 fatty acid levels
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DOI：
10.1016/j.placenta.2018.07.016
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发表时间：
2018-09-01
期刊：
影响因子：
3.8
通讯作者：
O'Tierney-Ginn, Perrie
中科院分区：
文献类型：
--
作者：
Alvarado, Fernanda L.;Calabuig-Navarro, Virtu;O'Tierney-Ginn, Perrie
简介：与瘦女性相比，肥胖女性的胎盘具有更高的脂质含量。我们先前已经证明，在中西部人群（俄亥俄州）中，超重和肥胖妇女补充omega-3脂肪酸可降低胎盘酯化途径和总脂质含量。我们假设，胎盘脂质积累和炎症在生活在高omega-3摄入地区（如夏威夷）的瘦女性和肥胖女性之间是相似的。方法：来自檀香山夏威夷的55名健康、正常葡萄糖耐受的女性，根据孕前BMI分为瘦体重和瘦体重。在计划足月剖腹产时，招募体重指数< 25 kg/m2（n=29）和肥胖（体重指数> 30 kg/m2（n=26））的受试者。通过质谱分析母体血浆DHA水平。使用qPCR测量胎盘组织中涉及脂肪酸氧化和酯化的关键基因的表达。通过Folch方法从胎盘组织中提取总脂质。TNF-α浓度测定酶联免疫吸附试验在胎盘lysated.Results：DHA水平较高，瘦妇女相比，肥胖妇女（P=0.02）。然而，夏威夷肥胖妇女的DHA水平比夏威夷肥胖妇女高8倍（P= 0.05）。此外，TNF-alpha胎盘裂解物瘦和肥胖women.Conclusions之间没有什么不同：虽然肥胖的妇女在夏威夷有较低的DHA水平相比，他们的瘦同行，这些水平仍然超过8倍高肥胖的俄亥俄州妇女。这些相对较高的血浆ω-3水平在夏威夷的肥胖妇女可能会抑制胎盘脂质酯化/储存和炎症，以相同的水平瘦妇女，如前所述，在体外。
Introduction: Placentas of obese women have higher lipid content compared to lean women. We have previously shown that supplementation of overweight and obese women with omega-3 fatty acids decreases placental esterification pathways and total lipid content in a mid-western population (Ohio). We hypothesized that placental lipid accumulation and inflammation would be similar between lean and obese women living in a region of high omega-3 intake, such as Hawaii.Methods: Fifty-five healthy, normal glucose tolerant women from Honolulu Hawaii, dichotomized based on pre-pregnancy BMI into lean (BMI < 25 kg/m(2), n=29) and obese (BMI > 30 kg/m(2), n=26), were recruited at scheduled term cesarean delivery. Maternal plasma DHA levels were analyzed by mass spectrometry. Expression of key genes involved in fatty acid oxidation and esterification were measured in placental tissue using qPCR. Total lipids were extracted from placental tissue via the Folch method. TNF-alpha concentration was measured by enzyme-linked immunosorbent assay in placental lysates.Results: DHA levels were higher in lean women compared to obese women (P=0.02). However, DHA levels in obese women in Hawaii were eight times higher compared to obese Ohioan women (P= 0.05) between lean and obese women in Hawaii. Furthermore, TNF-alpha placental lysates were not different between lean and obese women.Conclusions: Though obese women in Hawaii have lower DHA levels compared to their lean counterparts, these levels remain over eight times as high as obese Ohioan women. These relatively high plasma omega-3 levels in obese women in Hawaii may suppress placental lipid esterification/storage and inflammation to the same levels of lean women, as seen previously in vitro.