Thyroid hormone effects on androgen receptor messenger RNA expression in rat Sertoli and peritubular cells
Thyroid hormone effects on androgen receptor messenger RNA expression in rat Sertoli and peritubular cells
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DOI：
10.1677/joe.0.1560043
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发表时间：
1998-01-01
影响因子：
4
通讯作者：
Cooke, PS
中科院分区：
文献类型：
--
作者：
Arambepola, NK;Bunick, D;Cooke, PS
生后支持细胞成熟的特征是雄激素受体（AR）表达的显著升高，其在出生和成年之间增加数倍。由于3，3 '，5-三碘甲状腺原氨酸（T-3）和FSH均能调节Sertoli细胞的增殖和分化，我们测定了T-3和FSH对培养的5日龄大鼠Sertoli细胞AR mRNA表达的影响。这些培养物含有5-9%的管周细胞，其也表达AR mRNA。为了确保所观察到的T-3反应没有导致管周细胞，我们研究了T-3对AR mRNA表达在培养的20天龄的支持细胞（其中包含最小的管周污染）和管周细胞，并测量甲状腺激素受体（TR）mRNA表达在这两种细胞类型。将来自5日龄和20日龄大鼠睾丸的支持细胞在单独的无血清培养基（对照）或含有绵羊FSH（100 ng/ml）和/或T-3（100 nM）的无血清培养基中生长4天。从20日龄大鼠睾丸中纯化的管周细胞在含血清培养基中生长8天。将这些细胞以1：4分开，并再生长8天，最后4天在有或没有T-3的无血清培养基中生长。用北方印迹法测定所有培养物中TR和AR mRNA水平。培养5天和20天的支持细胞AR mRNA水平显著高于对照组（P
Postnatal Sertoli cell maturation is characterized by a pronounced rise in androgen receptor (AR) expression which increases several fold between birth and adulthood. Since both 3,3',5-triiodothyronine (T-3) and FSH regulate Sertoli cell proliferation and differentiation, we :have determined the effects of T-3 and FSH on AR mRNA expression in cultured Sertoli cells from 5-day-old rats. These cultures contain 5-9% peritubular cells, which also express AR mRNA. To insure that the observed T-3 responses did not result from peritubular cells, we examined T-3 effects on AR mRNA expression in cultured 20-day-old Sertoli cells (which contain minimal peri-tubular contamination) and peritubular cells, and measured thyroid hormone receptor (TR) mRNA expression in both of these cell types. Sertoli cells from 5- and 20-day-old rat testes were grown in serum-free medium alone (controls) or with ovine FSH (100 ng/ml) and/or T-3 (100 nM) for 4 days. Peritubular cells purified from 20-day-old rat testes were grown in serum-containing medium for 8 days. These cells were split 1:4, and grown an additional 8 days, the last 4 days in serum-free medium with or without T-3. TR and AR mRNA levels in all cultures were determined by Northern blotting. AR mRNA levels in 5- and 20-day-old cultured Sertoli cells were significantly (P