Isolation and characterization of microsatellite markers in Dysosma versipellis (Berberidaceae), a rare endemic from China
Isolation and characterization of microsatellite markers in Dysosma versipellis (Berberidaceae), a rare endemic from China
复制标题
中国罕见特有种八角藻（小檗科）微卫星标记的分离和鉴定
DOI：
10.1007/s10592-007-9393-0
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Fu, Cheng-Xin
中科院分区：
文献类型：
--
作者：
Guan, Bi-Cai;Qiu, Ying-Xiong;Fu, Cheng-Xin
八角莲(Dysosma Versiellis)是一种重要的濒危药用植物。在这里，我们用一种改进的生物素捕获法分离了9个多态的微卫星座位。我们分离的座位为SSR标记提供了每个座位2-7个等位基因的多态。预期杂合度为0.507~0.864，观测杂合度为0.360~0.720。这些标记将成为分析白粉虫种群遗传结构和交配系统等问题的有用工具。
Dysosma versipellis (Berberidaceae) is an important threatened medicinal plant (TMP) species. Here we isolated nine polymorphic microsatellite loci from D. versipellis using a modified biotin-capture method. Our isolated loci provided SSR markers with polymorphism of 2–7 alleles per locus. The expected and observed heterozygosities ranged from 0.507 to 0.864 and from 0.360 to 0.720, respectively. These markers would be the useful tools for analyzing questions concerning population genetic structure and mating system of D. versipellis.