Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China.
Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China.
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DOI：
10.1186/1743-422x-8-73
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发表时间：
2011-02-19
期刊：
影响因子：
4.8
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Hao X;Lu Z;Kuang W;Sun P;Fu Y;Wu L;Zhao Q;Bao H;Fu Y;Cao Y;Li P;Bai X;Li D;Liu Z
猪繁殖与呼吸综合征病毒（PRRSV）表现出广泛的遗传变异。 2006 年爆发的高致病性 PRRS 促使我们调查中国 PRRSV 遗传多样性的程度。为此，我们分析了98个中国PRRSV分离株的Nsp2和ORF7基因序列。初步分析，Nsp2蛋白30个氨基酸缺失的高致病性PRRSV株是我国流行的优势病毒。基于ORF7序列的进一步分析显示，所有中国分离株均分为5个亚组，且高致病性PRRSV与MLV或CH-1R疫苗有远缘关系，引发了人们对这些疫苗功效的怀疑。 ORF7 序列数据还显示，中国 PRRSV 的地理或时间起源与异质性之间没有明显关联。这些发现增强了我们对中国PRRSV分离株遗传特征的了解，并可能有助于制定中国监测和控制PRRSV的有效策略。
Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits extensive genetic variation. The outbreak of a highly pathogenic PRRS in 2006 led us to investigate the extent of PRRSV genetic diversity in China. To this end, we analyzed the Nsp2 and ORF7 gene sequences of 98 Chinese PRRSV isolates. Preliminary analysis indicated that highly pathogenic PRRSV strains with a 30-amino acid deletion in the Nsp2 protein are the dominant viruses circulating in China. Further analysis based on ORF7 sequences revealed that all Chinese isolates were divided into 5 subgroups, and that the highly pathogenic PRRSVs were distantly related to the MLV or CH-1R vaccine, raising doubts about the efficacy of these vaccines. The ORF7 sequence data also showed no apparent associations between geographic or temporal origin and heterogeneity of PRRSV in China. These findings enhance our knowledge of the genetic characteristics of Chinese PRRSV isolates, and may facilitate the development of effective strategies for monitoring and controlling PRRSV in China.