A sex-determining region on the Y chromosome controls the sex-reversal ratio in interspecific hybrids between Oryzias curvinotus females and Oryzias latipes males
A sex-determining region on the Y chromosome controls the sex-reversal ratio in interspecific hybrids between Oryzias curvinotus females and Oryzias latipes males
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DOI：
10.1038/hdy.2009.114
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发表时间：
2010-02-01
期刊：
影响因子：
3.8
通讯作者：
Hamaguchi, S.
中科院分区：
文献类型：
--
作者：
Kato, M.;Takehana, Y.;Hamaguchi, S.
青鳉和青鳉是近缘的青鳉属物种，它们的同源Y染色体上具有共同的性别决定基因DMY。我们以前报道过，性反转XY雌性产生于O。雌性和O. latipes雄性（Hd-rR近交系）。在这项研究中，我们使用HNI近交系雄性O。latipes与O. curvinotus女性，并发现所有的XY杂种发展为男性。为了定位这种株系特异性XY性逆转的因子，O。用两个Y同源株（HNI.YHd-rR和Hd-rR.Y-HNI）和一个重组同源株（Hd-rR.Y（HNI）rr）与弯鼻蝠雌性交配。HNI.YHd-rR在XY杂种中产生性反转雌性，而在来自Hd-rR.Y-HNI和Hd-rR.Y（HNI）rr的XY杂种中没有获得性反转雌性，这表明Y染色体上的一个小区域（包括DMY）负责XY性反转。性反转的杂交体只产生在来自Hd-RR菌株的Y染色体区域的存在下，这表明特异于Hd-RR Y染色体区域的错义或调控突变诱导性反转。Heredity（2010）104，191-195; doi：10.1038/hdy.2009.114; 2009年9月16日在线发表
Oryzias latipes and Oryzias curvinotus are closely related medaka species that have the common sex-determining gene, DMY, on their homologous Y chromosomes. We previously reported that sex-reversed XY females were produced in hybrids between O. curvinotus females and O. latipes males (Hd-rR inbred strain). In this study we used HNI inbred strain males of O. latipes for mating with O. curvinotus females, and found that all the XY hybrids developed as males. To map the factor responsible for this strain-specific XY sex reversal, O. curvinotus females were mated with two Y-congenic strains (HNI.YHd-rR and Hd-rR.Y-HNI) and a recombinant congenic strain (Hd-rR.Y(HNI)rr). HNI.YHd-rR produced sex-reversed females in the XY hybrids, whereas no sex-reversed females were obtained in the XY hybrids from Hd-rR.Y-HNI and Hd-rR.Y(HNI)rr, demonstrating that a small region on the Y chromosome, which includes DMY, is responsible for the XY sex reversal. Sex-reversed hybrids were only produced in the presence of the Y-chromosomal region derived from the Hd-rR strain, suggesting that missense or regulatory mutations specific to the Hd-rR Y-chromosomal region induce the sex reversal. Heredity (2010) 104, 191-195; doi:10.1038/hdy.2009.114; published online 16 September 2009