Biodistribution and toxicity of orally administered poly(lactic-co-glycolic) acid nanoparticles to F344 rats for 21 days
Biodistribution and toxicity of orally administered poly(lactic-co-glycolic) acid nanoparticles to F344 rats for 21 days
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DOI：
10.2217/nnm-2016-0022
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发表时间：
2016-07-01
期刊：
影响因子：
5.5
通讯作者：
Sabliov, Cristina M.
中科院分区：
文献类型：
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作者：
Navarro, Sara M.;Morgan, Timothy W.;Sabliov, Cristina M.
目的：F344大鼠口服PLGA（聚乳酸-羟基乙酸共聚物）和表面改性PLGA壳聚糖（PLGA/Chi）纳米粒（NPs）7、14和21天，定量生物分布并评估其毒性。材料和方法：在F344大鼠组织中追踪荧光纳米颗粒，并通过碱性磷酸酶和丙氨酸转氨酶水平以及组织样品的组织学检查来评价毒性。结果：PLGA和PLGA/Chi的生物分布相似，在肠道和肝脏中发现的量最高。治疗组大鼠碱性磷酸酶显著升高。在肠和肝脏中检测到轻度组织学差异。结论：PLGA和PLGA/Chi纳米粒表现相似，在肝脏和肠道中表现出最小的毒性，但在肾脏、肺和脑中没有毒性。
Aim: Quantify the biodistribution and assess the toxicity of PLGA (poly-lactic-co-glycolic acid) and surface-modified PLGA chitosan (PLGA/Chi) nanoparticles (NPs) orally administered for 7, 14 and 21 days to F344 rats. Materials & methods: Fluorescent NPs were tracked in F344 rat tissues, and toxicity was evaluated by alkaline phosphatase and alanine transaminase levels, and by histologic examination of tissue samples. Results: Biodistribution of PLGA and PLGA/Chi were similar, with highest amounts found in the intestine and liver. Alkaline phosphatase increased significantly in treated rats. Mild histological differences were detected in the intestine and liver. Conclusion: PLGA and PLGA/Chi NPs behaved similarly presenting minimal toxicity in the liver and intestine, but not in kidney, lung and brain.