Molecular Detection of Human Periodontal Pathogens in Oral Swab Specimens from Dogs in Japan
Molecular Detection of Human Periodontal Pathogens in Oral Swab Specimens from Dogs in Japan
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DOI：
10.1177/089875641102800204
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发表时间：
2011-06-01
影响因子：
1
通讯作者：
Asai, Fumitoshi
中科院分区：
文献类型：
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作者：
Kato, Yukio;Shirai, Mitsuyuki;Asai, Fumitoshi
牙周病已知是人类的主要疾病，在狗中也很常见。本研究的目的是利用从 26 只宠物狗身上采集的口腔拭子标本来分析牙周炎相关细菌种类的分布。动物牙龈生物古莱卟啉单胞菌的分布，以及10种人类牙周炎相关细菌的分布，包括牙龈卟啉单胞菌、齿垢密螺旋体、连翘坦氏菌、赭二氧化碳噬菌体、痰二氧化碳嗜二氧化碳菌、中间普雷沃菌、黑普氏菌、放线菌聚集菌、直肠弯曲杆菌和腐蚀艾肯氏菌通过聚合酶链反应与物种特异性引物组进行评估。几乎所有分析的狗中都检测到了古莱卟啉单胞菌、连翘坦纳菌和直肠弯曲杆菌，所有这些都应被视为狗口腔菌群的常见成员。然后，对拭子标本中的卟啉单胞菌属进行了分离和鉴定。从 22 只狗身上分离出 35 株菌株，广泛的聚合酶链反应和测序方法显示，其中大约 70% 是古拉卟啉单胞菌。相比之下，牙龈卟啉单胞菌的频率极低。这些发现表明宠物狗中存在特定的牙周炎相关病原体，尤其是古拉卟啉单胞菌。兽医牙科杂志 28(2) 84 - 89, 2011
Periodontal diseases are known to be major diseases in humans, and are also common in dogs. The purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. The distribution of an animal gingival organism Porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans, Campylobacter rectus, and Eikenella corrodens, were evaluated by polymerase chain reaction with species-specific sets of primers. Porphyromonas gulae, Tannerella forsythia and Campylobacter rectus were detected in almost all dogs analyzed, all of which should be regarded as common members of oral flora in dogs. Then, isolation and identification of the Porphyromonas species in swab specimens were performed. There were 35 strains isolated from 22 dogs, and broad-range polymerase chain reaction and sequencing methods revealed that approximately 70 % of them were Porphyromonas gulae. In contrast, the frequency of Porphyromonas gingivalis was extremely low. These findings indicate the presence of specific periodontitis-related pathogens in pet dogs, especially Porphyromonas gulae. J Vet Dent 28(2) 84 - 89, 2011