Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells
Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells
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DOI：
10.1016/j.celrep.2021.109812
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发表时间：
2021-10-12
期刊：
影响因子：
8.8
通讯作者：
Surani, M. Azim
中科院分区：
文献类型：
--
作者：
Kobayashi, Toshihiro;Castillo-Venzor, Aracely;Surani, M. Azim
兔胚胎在原肠胚形成时发育为双层盘，与人类和大多数其他哺乳动物一样，而啮齿动物发育为卵圆柱体。对哺乳动物胚胎的系统研究表明，原始生殖细胞（PGCs）起源于原肠胚形成期。在这里，我们表明，兔PGC（rbPGC）规范发生在后上胚层在原肠胚形成的发病。使用新衍生的兔多能干细胞，我们在体外用WNT和BMP形态发生剂显示了rbPGC样细胞的稳健和快速诱导，这揭示了SOX 17在几种非啮齿类哺乳动物中作为rbPGC命运的关键调节剂。我们认为，发展作为一个双层光盘是一个至关重要的决定因素的PGC监管机构，无论高度多样化的发展胚外组织，包括羊膜。我们建议，对妊娠期短、窝仔数大、原肠胚形成先于着床的兔进行研究，可以显著促进早期哺乳动物发育的进展。
Rabbit embryos develop as bilaminar discs at gastrulation as in humans and most other mammals, whereas rodents develop as egg cylinders. Primordial germ cells (PGCs) appear to originate during gastrulation according to many systematic studies on mammalian embryos. Here, we show that rabbit PGC (rbPGC) specification occurs at the posterior epiblast at the onset of gastrulation. Using newly derived rabbit pluripotent stem cells, we show robust and rapid induction of rbPGC-like cells in vitro with WNT and BMP morphogens, which reveals SOX17 as the critical regulator of rbPGC fate as in several non-rodent mammals. We posit that development as a bilaminar disc is a crucial determinant of the PGC regulators, regardless of the highly diverse development of extraembryonic tissues, including the amnion. We propose that investigations on rabbits with short gestation, large litters, and where gastrulation precedes implantation can contribute significantly to advances in early mammalian development.