CNBP regulates forebrain fon-nation at organogenesis stage in chick embryos
CNBP regulates forebrain fon-nation at organogenesis stage in chick embryos
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DOI：
10.1016/j.ydbio.2006.03.012
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发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Li, Yi-Ping
中科院分区：
文献类型：
--
作者：
Abe, Yoko;Chen, Wei;Li, Yi-Ping
我们最近证明，细胞核酸结合蛋白（CNBP）（-/-）小鼠胚胎由于在原肠胚形成前阶段缺乏前内脏内胚层（AVE）的适当形态发生运动而表现出前脑截短（Chen，W.，梁玉，邓小平，Shimizu，K.，Ashique，上午，Li，E.，李延平，2003.锌指蛋白CNBP是小鼠前脑形成所必需的，Development 130，1367-1379）。然而，CNBP在小鼠前脑中的表达模式表明，CNBP可能在前脑发育过程中有更直接的影响。我们的数据表明，CNBP在与小鼠胚胎相当的早期鸡胚组织中表达。使用RNAi沉默和逆转录病毒错误表达的方法相结合，我们研究了CNBP在器官发生过程中鸡前脑的规范/发育中的时间功能。CNBP表达的沉默导致鸡胚前脑截短，BF-1，Six 3和Hesx 1表达缺失，但不表达Otx 2。CNBP的错误表达诱导了BE-1、Six 3和Hesx 1在后脑中的表达，但不诱导Otx 2的表达。这些结果提供了新的见解CNBP在器官发生过程中作为前脑形成的调节器和一些喙头转录因子的功能。此外，CNBP和Otx 2可能在两个平行的通路中作为前脑形成的调节剂发挥作用。这些新的见解CNBP的功能强调了重要的作用，CNBP在前脑的形成过程中鸡胚器官发生。(c)2006年爱思唯尔公司All rights reserved.
We recently demonstrated that Cellular Nucleic acid Binding Protein (CNBP)(-/-) mouse embryos exhibit forebrain truncation due to a lack of proper morphogenetic movements of the anterior visceral endoderm (AVE) during pre-gastrulation stage (Chen, W., Liang, Y., Deng, W., Shimizu, K., Ashique, A.M., Li, E., Li, Y.P., 2003. The zinc-finger protein CNBP is required for forebrain formation in the mouse, Development 130, 1367-1379). However, CNBP expression pattern in the mouse forebrain suggests that CNBP may have more direct effects during forebrain development. Our data show that CNBP is expressed in tissues of early chick embryo that are the equivalent to the mouse embryo. Using a combination of RNAi-silencing and Retrovirus-misexpression approaches, we investigated the temporal function of CNBP in the specification/development of the chick forebrain during organogenesis. The silencing of CNBP expression resulted in forebrain truncation and the absence of BF-1, Six3 and Hesx1 expression, but not Otx2 in chick embryos. Misexpression of CNBP induced the expression of BE-1, Six3 and Hesx1 in the hindbrain, but not the expression of Otx2. These results offer novel insights into the function of CNBP during organogenesis as the regulator of forebrain formation and a number of rostral head transcription factors. Moreover, CNBP and Otx2 may play roles as regulators of forebrain formation in two parallel pathways. These new insights into CNBP functions underscore the essential role of CNBP in forebrain formation during chick embryo organogenesis. (c) 2006 Elsevier Inc. All rights reserved.