BmCF1, a Bombyx mori RXR-type receptor related to the Drosophila ultraspiracle.
BmCF1, a Bombyx mori RXR-type receptor related to the Drosophila ultraspiracle.
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DOI：
10.1006/jmbi.1994.1306
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发表时间：
1994-05
影响因子：
5.6
通讯作者：
G. Tzertzinis;A. Małecki;F. Kafatos
中科院分区：
文献类型：
--
作者：
G. Tzertzinis;A. Małecki;F. Kafatos
采用 PCR 方法从家蚕中获得卵巢 cDNA 克隆，编码 50 kDa 蛋白 (BmCF1)，属于核激素受体 RXR 亚家族，与果蝇 ultraspiracle 基因编码的 CF1/USP 蛋白 (DmCF1) 最相似。 DNA 结合域的相似性较高，配体结合域的相似性中等，但在 N 端（推定的激活剂 A/B 域）不相似。与可用 RXR 序列的蛋白质序列比较表明，尽管昆虫 USP 样序列彼此之间的相关性比脊椎动物 RXR 的相关性更高，但它们之间的相似性低于脊椎动物 RXR 的情况。一致地观察到两个不同的 BmCF1 同源转录物，并且表明蛾中存在选择性剪接或第二个 RXR 基因。这些转录本分布广泛，表明其在多个发育阶段都有功能，就像果蝇超气门的情况一样。
A PCR approach has been used to obtain an ovarian cDNA clone from the silkmoth Bombyx mori, encoding a 50 kDa protein (BmCF1) that belongs to the RXR subfamily of nuclear hormone receptors and is most similar to the CF1/USP protein (DmCF1) encoded by the ultraspiracle gene of Drosophila. The similarity is high in the DNA-binding and moderately so in the ligand-binding domains, although not in the N-terminal, putatively activator A/B domain. Protein sequence comparisons with the available RXR sequences indicate that although insect USP-like sequences are more related to each other than to vertebrate RXRs, their inter se similarities are lower than in the case of the vertebrate RXRs. Two distinct BmCF1-homologous transcripts are observed consistently, and are indicative either of alternative splicing or of the existence of a second RXR gene in the moth. The transcripts are widely distributed, suggesting functions at multiple developmental stages, as in the case of Drosophila ultraspiracle.