Mutants of the Yarrowia lipolytica PEX23 gene encoding an integral peroxisomal membrane peroxin mislocalize matrix proteins and accumulate vesicles containing peroxisomal matrix and membrane proteins
Mutants of the Yarrowia lipolytica PEX23 gene encoding an integral peroxisomal membrane peroxin mislocalize matrix proteins and accumulate vesicles containing peroxisomal matrix and membrane proteins
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DOI：
10.1091/mbc.11.1.141
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发表时间：
2000-01-01
影响因子：
3.3
通讯作者：
Rachubinski, RA
中科院分区：
文献类型：
--
作者：
Brown, TW;Titorenko, VI;Rachubinski, RA
Pex突变体在过氧化物酶体组装上有缺陷。酵母多脂耶氏菌的突变株pex23-1缺乏形态学上可识别的过氧化物酶体，并且优先将所有过氧化物酶体基质蛋白错定位于细胞质。Pex23菌株积累了含有过氧化物酶体基质和膜蛋白的囊泡结构。PEX23基因通过PEX23 -1菌株的功能互补分离得到，编码418个氨基酸（47,588 Da）的蛋白Pex23p。Pex23p与酿酒酵母（Saccharomyces cerevisiae）的两种假设蛋白具有高度的序列相似性。Pex23p是一种完整的过氧化物酶体膜蛋白，完全或几乎完全与细胞质隔离。在含葡萄糖培养基中生长的细胞中检测到低水平的Pex23p，在含油酸的培养基中生长，其水平显著提高，油酸的代谢需要完整的过氧化物酶体。
pex mutants are defective in peroxisome assembly. The mutant strain pex23-1 of the yeast Yarrowia lipolytica lacks morphologically recognizable peroxisomes and mislocalizes all peroxisomal matrix proteins investigated preferentially to the cytosol. pex23 strains accumulate vesicular structures containing both peroxisomal matrix and membrane proteins. The PEX23 gene was isolated by functional complementation of the pex23-1 strain and encodes a protein, Pex23p, of 418 amino acids (47,588 Da). Pex23p exhibits high sequence similarity to two hypothetical proteins of the yeast Saccharomyces cerevisiae. Pex23p is an integral membrane protein of peroxisomes that is completely, or nearly completely, sequestered from the cytosol. Pex23p is detected at low levels in cells grown in medium containing glucose, and its levels are significantly increased by growth in medium containing oleic acid, the metabolism of which requires intact peroxisomes.