Functional analysis of the proteases overexpressed during the invasive and parasitic stages of Cryptocaryon irritans and their potential as vaccine antigens
Functional analysis of the proteases overexpressed during the invasive and parasitic stages of Cryptocaryon irritans and their potential as vaccine antigens
复制标题
刺激白点虫侵入和寄生阶段过度表达的蛋白酶的功能分析及其作为疫苗抗原的潜力
DOI：
10.1016/j.aquaculture.2021.736657
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发表时间：
2021
期刊：
影响因子：
4.5
通讯作者：
TomoyoshiYoshinaga
中科院分区：
文献类型：
--
作者：
Yuho Watanabe;Kosuke Zenke;Naoki Itoh;TomoyoshiYoshinaga
在本研究中，我们旨在利用RNAi分析隐核虫感染过程中高表达的蛋白酶在隐核体刺激体的热体（侵入阶段）和营养体（寄生阶段）阶段的功能。我们还通过用重组蛋白酶感染虎河豚（Takifugu rubripes），分析了这些蛋白酶作为寄生虫疫苗接种候选抗原的潜力。与对照组相比，针对四种蛋白酶（包括肽酶）基因的RNAi减少了感染并在完全发育后离开鱼的寄生虫数量。然后纯化四种重组蛋白酶并作为候选疫苗接种抗原进行评估。从用 C 免疫的鱼中回收的寄生虫数量。刺激物重组蛋白低于从对照鱼中回收的蛋白；当使用重组半胱氨酸蛋白酶时，这种差异具有统计学意义。这些结果表明 C.在乳体和营养体阶段过度表达的刺激物蛋白酶对于感染（包括入侵、食物摄取、消化和发育）很重要，并且可以作为开发隐核虫病疫苗的目标。
In this study, we aimed to analyze the functions of highly expressed proteases at the theront (invasive stage) and trophont (parasitic stage) phases ofCryptocaryon irritansduring the infection process using RNAi. We also analyzed the potential of these proteases as candidate antigens for vaccination against the parasite by infecting tiger pufferfish (Takifugu rubripes) with the recombinant proteases. Compared with the control group, RNAi against four proteases (including peptidases) genes reduced the number of parasites that infected and left the fish after complete development. The four recombinant proteases were then purified and evaluated as candidate vaccination antigens. The number of parasites recovered from fish immunized with theC. irritansrecombinant proteins was lower than that recovered from control fish; this difference was statistically significant when a recombinant cysteine protease was used. These results suggest that theC. irritansproteases overexpressed at the theront and trophont stages are important for infection (including invasion, food uptake, digestion, and development) and can be targeted for the development of vaccines against cryptocaryoniasis.