Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)
Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)
复制标题
DOI：
10.1007/s13258-010-0124-4
复制
发表时间：
2011-04-01
期刊：
影响因子：
2.1
通讯作者：
Yang, Yonghua
中科院分区：
文献类型：
--
作者：
Fang, Rongjun;Hu, Dongqing;Yang, Yonghua
根据桑葚表达序列标签（ESTs），克隆了桑葚CaM基因的全长cDNA序列，命名为MCaM-3（GenBank登录号：GQ 303247）。序列分析表明，MCaM-3全长951 bp，编码149个氨基酸，预测分子量为16.85kD，等电点为3.95。在线SMART分析表明，MCaM-3蛋白具有四个EFh功能结构域，可以结合钙离子。研究了MCaM-3在桑葚和花不同发育时期以及不同组织中的表达水平。结果表明，MCaM-3基因在成熟组织中表达量最高，如成熟雌花和顶极叶，在低温、干旱和盐胁迫条件下，MCaM-3基因的表达量显著高于正常生长环境。该研究有助于我们了解桑葚抗病功能基因的抗病机理。
A full-length cDNA sequence encoding CaM from mulberry, which we designated MCaM-3 (GenBank accession No: GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed that MCaM-3 is 951 base pairs in length, encoding 149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium. The expression level of MCaM-3at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry.