Requirement of miR-144 in CsA Induced Proliferation and Invasion of Human Trophoblast Cells by Targeting Titin
Requirement of miR-144 in CsA Induced Proliferation and Invasion of Human Trophoblast Cells by Targeting Titin
复制标题
CsA 通过靶向肌联蛋白诱导人滋养层细胞增殖和侵袭时需要 miR-144
DOI：
10.1002/jcb.24710
复制
发表时间：
2014-04-01
影响因子：
4
通讯作者：
Wan, Shumei
中科院分区：
文献类型：
--
作者：
Liang, Ying;Lin, Qinlu;Wan, Shumei
microRNA（miRNAs）是一类内源性的19-25个核苷酸的非编码单链RNA，通过阻断mRNA的翻译或降低mRNA的稳定性来调节基因的表达。在这项研究中，通过CsA（环孢菌素A）的处理，我们发现，miR-144的表达水平下降，而肌联蛋白mRNA的表达水平在人类滋养层细胞（HT）中增加，并确定肌联蛋白作为一种新的直接靶点的miR-144。过表达miR-144可抑制titin及其下游信号分子如p-ERK 1/2和MMP 2/9的表达，并减弱细胞增殖和侵袭。titin的强制表达可以部分地挽救miR-144在细胞中的抑制作用。这些研究结果有助于阐明miR-144通过miR-144/titin轴调控HT细胞增殖和侵袭的作用和机制，miR-144可能成为HT潜在的治疗靶点。J.细胞。115：690-696，2014. (c)2013 Wiley Periodicals，Inc.
MicroRNAs (miRNAs) are endogenous 19-25 nucleotide noncoding single-stranded RNAs that regulate gene expression by blocking the translation or decreasing the stability of mRNAs. In this study, with the treatment of CsA (Cyclosporin A), we showed that miR144 expression levels were decreased while titin mRNA expression levels were increased in human trophoblast (HT) cells, and identified titin as a novel direct target of miR-144. Overexpression of miR-144 suppressed titin and its downstream signaling molecule such as p-ERK1/2 and MMP2/9 expression, and attenuated cell proliferation and invasion. Forced expression of titin can partly rescue the inhibitory effect of miR-144 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-144 in regulating HT cells proliferation and invasion via miR-144/titin axis, and miR-144 may serve as a potential therapeutic target in HT in the future. J. Cell. Biochem. 115: 690-696, 2014. (c) 2013 Wiley Periodicals, Inc.