Possible role of PEPT1 in gastrointestinal hormone secretion
Possible role of PEPT1 in gastrointestinal hormone secretion
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DOI：
10.1016/j.bbrc.2005.08.259
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发表时间：
2005-11-04
影响因子：
3.1
通讯作者：
Seino, S
中科院分区：
文献类型：
--
作者：
Matsumura, K;Miki, T;Seino, S
来源于膳食的寡肽可刺激多种胃肠激素的分泌，但其作用机制尚不清楚。在这项研究中，我们发现，寡肽转运体1(PEPT1)在STC-1细胞(一种小鼠肠道内分泌细胞系)中的转基因能够以一种pH依赖的方式引起二肽刺激的激素分泌。膜电位测定表明，转导PEPT1的STC-1细胞可被甘氨酰甘氨酸去极化，但不能被甘氨酸单体去极化。甘氨酰-甘氨酸刺激可诱导PEPT1转基因STC-1细胞内钙离子浓度升高。钙通道阻断剂硝苯地平可阻断甘氨酰-甘氨酸诱导的分泌，提示这种分泌是由L型电压依赖性钙通道的钙内流触发的。这些数据表明，PEPT1介导了寡肽诱导的肠内分泌细胞的激素分泌。(C)2005 Elsevier Inc.保留所有权利。
Oligopeptides originating from ingested meal stimulate the secretion of various gastrointestinal hormones, but the mechanism is unknown. In this study, we show that transfection of oligopeptide transporter 1 (PEPT1) in STC-1 cells, a murine enteroendocrine cell line, evokes di-peptide-stimulated hormone secretion in a pH-dependent manner. Measurement of membrane potentials shows that PEPT1- transfected STC-1 cells are depolarized by di-peptide glycyl-glycine but not by glycine monomer. Glycyl-glycine stimulation induces a rise in the intracellular calcium concentration in PEPT1-transfected STC-1 cells. The secretion induced by glycyl-glycine in PEPT1-transfected STC-1 cells was blocked by nifedipine, a Ca2+ channel blocker, suggesting that the secretion is triggered by Ca2+ influx through L-type voltage-dependent Ca2+ channels. These data suggest that PEPT1 mediates oligopeptide-induced hormone secretion in enteroendocrine cells. (c) 2005 Elsevier Inc. All rights reserved.