Effect of zinc supplementation on plasma IL-6 and MCP-1 production and NK cell function in healthy elderly: Interactive influence of+647 MT1a and-174 IL-6 polymorphic alleles
Effect of zinc supplementation on plasma IL-6 and MCP-1 production and NK cell function in healthy elderly: Interactive influence of+647 MT1a and-174 IL-6 polymorphic alleles
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DOI：
10.1016/j.exger.2007.12.003
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发表时间：
2008-05-01
影响因子：
3.9
通讯作者：
Facchini, Andrea
中科院分区：
文献类型：
--
作者：
Mariani, Erminia;Neri, Simona;Facchini, Andrea
促炎症细胞因子反应和NK活性由锌离子的可获得性控制，锌离子的细胞内运输受到金属硫蛋白的调节。为了密切研究循环锌在调节衰老过程中的免疫反应、Th2/Th1平衡以及最终在全身低度炎症可逆性中的重要性，我们评估了补充天冬氨酸锌后健康低度炎症老年人群血浆IL-6和MCP-1浓度和NK裂解活性的变化。此外，我们的目的是强调循环锌增量、免疫学参数变化和+647 MT1a和-174 IL-6多态等位基因之间的潜在交互作用。来自ZINCAGE队列的39名健康个体(60-83岁)(先前被分型为+647 MT1a和-174 IL-6多态)被提供天冬氨酸锌。在补充前和补充后采集血样进行基础实验室检测(循环锌、白蛋白和C反应蛋白)和免疫学检测(血浆IL-6和MCP-1和NK细胞溶解活性)。低循环锌或接近正常循环锌的受试者补锌可增加循环锌离子浓度，调节血浆IL-6、MCP-1和NK细胞的溶解活性。补充后MT1a和IL-6基因的多态等位基因对锌、IL-6、MCP-1和NK活性的交互作用被证实，这表明遗传背景是识别可以利用治疗干预的受试者组的决定因素之一。(C)2007 Elsevier Inc.保留所有权利。
Pro-inflammatory cytokine response and NK activity are controlled by the availability of zinc ion, whose intra-cellular transport is regulated by metallothioneins.In order to closely examine the importance of circulating zinc in the modulation of immune response during ageing, in the balance of Th2/Th1 equilibrium and finally in the reversibility of systemic low grade inflammation, we evaluated the changes occurring in plasma IL-6 and MCP-1 concentrations and NK lytic activity in a healthy low grade inflamed elderly population, following zinc-aspartate supplementation. In addition, we aimed to highlight the potential interaction among circulating zinc increments, changes in immunological parameters and +647 MT1a and -174 IL-6 polymorphic alleles.Thirty-nine healthy individuals (60-83 years) from the ZINCAGE cohort (previously typed for +647 MT1a and - 174 IL-6 polymorphisms) were supplied with zinc-aspartate. Blood samples collected before and after supplementation underwent basal laboratory determinations (circulating zinc, albumin and C-reactive protein) and immunological studies (plasma IL-6 and MCP-1 and NK lytic activity). Zinc supplementation in subjects with low or borderline-normal circulating zinc increased the concentration of this ion and modulated plasmatic IL-6 and MCP-1 as well as NK lytic activity. An interactive effect of polymorphic alleles of MT1a and IL-6 genes on zinc, IL-6, MCP-1 and NK activity was evidenced following supplementation, indicating the genetic background as one of the determinants for identifying groups of subjects that can take advantage of therapeutic intervention. (C) 2007 Elsevier Inc. All rights reserved.