Comprehensive epigenome and transcriptome analysis of carbon reserve remobilization in indica and japonica rice stems under moderate soil drying
Comprehensive epigenome and transcriptome analysis of carbon reserve remobilization in indica and japonica rice stems under moderate soil drying
复制标题
中度土壤干燥条件下籼粳稻茎碳储量再利用的表观基因组和转录组综合分析
DOI：
10.1093/jxb/eraa502
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发表时间：
2021
影响因子：
6.9
通讯作者：
Zhang Jianhua
中科院分区：
文献类型：
--
作者：
Wang Guanqun;Li Xiaozheng;Li Yongqiang;Ye Nenghui;Li Haoxuan;Zhang Jianhua
在花后阶段施加适度的土壤干燥(MD)显著改善了水稻茎中碳储量的再动员，从而增加了籽粒产量。然而，在MD下，碳储备重新动员的甲基组和转录组的轮廓是模糊的水稻和水稻茎。在这里，我们生成了DNA甲基组水稻和水稻茎的全基因组单碱基分辨率图谱。DNA甲基化水平较高，且与基因组大小呈正相关。MD处理对籼稻甲基化水平的变化影响较小。此外，差异甲基化区域的数量要比籼稻少得多，这表明在灌浆过程中存在品种特有的甲基化模式，以响应MD。参与淀粉降解过程的编码β-葡萄糖苷酶1的基因被低甲基化和上调，从而提高了MD处理下淀粉到蔗糖的转化率。此外，MYBS1基因的高表达反式激活了AMYC2/OsAMY2A在水稻和粳稻中的表达，从而促进了在MD下的淀粉降解。相反，MYB30的表达下调导致BMY5在两个品种中的表达增加。我们的发现破译了水稻和水稻茎DNA甲基化的动态，并提出了改善碳储备再动员的候选基因。
Moderate soil drying (MD) imposed at the post-anthesis stage significantly improves carbon reserve remobilization in rice stems, increasing grain yield. However, the methylome and transcriptome profiles of carbon reserve remobilization under MD are obscure inindicaandjaponicarice stems. Here, we generated whole-genome single-base resolution maps of the DNA methylome inindicaandjaponicarice stems. DNA methylation levels were higher inindicathan injaponicaand positively correlated with genome size. MD treatment had a weak impact on the changes in methylation levels inindica. Moreover, the number of differentially methylated regions was much lower inindica,indicating the existence of cultivar-specific methylation patterns in response to MD during grain filling. The gene encoding β-glucosidase 1, involved in the starch degradation process, was hypomethylated and up-regulated inindica, resulting in improved starch to sucrose conversion under MD treatment. Additionally, increased expression of MYBS1 transactivated the expression ofAMYC2/OsAMY2Ain bothindicaandjaponica, leading to enhanced starch degradation under MD. In contrast, down-regulated expression ofMYB30resulted in increased expression ofBMY5in both cultivars. Our findings decode the dynamics of DNA methylation inindicaandjaponicarice stems and propose candidate genes for improving carbon reserve remobilization.