Escherichia coli Strain Designed for Characterizing in Vivo Functions of Nicotinamide Adenine Dinucleotide Analogues
Escherichia coli Strain Designed for Characterizing in Vivo Functions of Nicotinamide Adenine Dinucleotide Analogues
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设计用于表征烟酰胺腺嘌呤二核苷酸类似物体内功能的大肠杆菌菌株
DOI：
10.1021/acs.orglett.9b00935
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Zhao Zongbao K
中科院分区：
文献类型：
--
作者：
Wang Lei;Liu Bin;Liu Yuxue;Sun Yue;Liu Wujun;Yu Dayu;Zhao Zongbao K
为有效地将烟酰胺腺嘌呤二核苷酸(NAD)类似物导入细胞，通过限制细胞外降解同时表达有效的NAD导入子，构建了无菌大肠埃希菌。烟酰胺次黄嘌呤二核苷酸被鉴定为NAD合成的抑制因子。烟酰胺胞嘧啶二核苷酸具有良好的生物相容性，被用来表征烟酰胺辅因子的生长依赖降解。
AnEscherichia colistrain was constructed for the efficient import of nicotinamide adenine dinucleotide (NAD) analogues into cells by limiting extracellular degradation while expressing an efficient NAD importer.In vivofunctions of three NAD analogues were characterized. Nicotinamide hypoxanthine dinucleotide was identified as an inhibitor of NAD synthesis. Nicotinamide cytosine dinucleotide had excellent biocompatibility and was used for characterizing a growth-dependent degradation ofin vivonicotinamide cofactors.