Cyclo-Oxygenase-2 Expression Is Associated With Vascular Endothelial Growth Factor C Expression and Lymph Node Metastasis in Oral Squamous Cell Carcinoma
Cyclo-Oxygenase-2 Expression Is Associated With Vascular Endothelial Growth Factor C Expression and Lymph Node Metastasis in Oral Squamous Cell Carcinoma
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DOI：
10.1016/j.joms.2013.04.015
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发表时间：
2013-10-01
影响因子：
1.9
通讯作者：
Chikazu, Daichi
中科院分区：
文献类型：
--
作者：
Kono, Michihide;Watanabe, Masato;Chikazu, Daichi
目的：口腔鳞状细胞癌（OSCC）的颈淋巴结转移被认为是一个预后不良的因素，尽管其机制尚不清楚。近年来，环氧合酶-2（考克斯-2）水平与血管内皮生长因子C（VEGF-C）和淋巴结转移密切相关。然而，在口腔鳞癌中还没有这种相关性的报道。因此，本研究的目的是探讨考克斯-2免疫组化表达与VEGF-C表达、组织病理学参数和淋巴结转移之间的关系。材料和方法：对60例口腔鳞癌组织进行淋巴管密度、VEGF-C和考克斯-2免疫组化检测。结果：淋巴结转移组VEGF-C、考克斯-2的表达及淋巴管密度均显著高于无转移组（P <0.01）。VEGF-C和考克斯-2的表达水平呈显著相关（r = 0.512; P <0.001）。考克斯-2表达与淋巴结转移（P = 0.004）和VEGF-C表达（P = 0.005）显著相关。单因素分析显示，较高的考克斯-2和VEGF-C表达水平损害了生存时间。多因素生存分析显示考克斯-2表达是影响OSCC患者预后的独立因素。结论：考克斯-2可上调VEGF-C的表达。高VEGF-C表达似乎促进肿瘤周围淋巴管生成。提示考克斯-2和VEGF-C对口腔鳞癌的淋巴结转移有促进作用。(C)2013年美国口腔颌面外科医师协会
Purpose: Cervical lymph node metastasis in oral squamous cell carcinoma (OSCC) is recognized as a poor prognostic factor, although its mechanism remains unclear. Recently, cyclo-oxygenase-2 (COX-2) level has been found to correlate highly with vascular endothelial growth factor C (VEGF-C) and lymph node metastasis, as in other solid tumors. However, there has been no report of this correlation in OSCC. Therefore, the aim of this study was to investigate whether COX-2 immunohistochemical expression in OSCC was associated with VEGF-C expression, histopathologic parameters, and lymph node metastasis.Materials and Methods: Lymphatic vessel density, VEGF-C, and COX-2 immunohistochemical expression were examined pathologically in 60 specimens of invasive OSCC. Relations of histopathologic parameters to lymph node metastasis were analyzed.Results: Expression levels of VEGF-C and COX-2 and lymphatic vessel density in the lymph node metastatic group were significantly higher than in the nonmetastatic group (P < .01). A significant correlation was found between the expression levels of VEGF-C and COX-2 (r = 0.512; P < .001). COX-2 expression was significantly related to lymph node metastasis (P = .004) and VEGF-C expression (P = .005). Univariate analysis showed that survival time was impaired by higher COX-2 and VEGF-C expression levels. Multivariate survival analysis showed that COX-2 expression was an independent prognostic factor.Conclusion: This study showed that VEGF-C expression was upregulated by COX-2 in OSCC. High VEGF-C expression appears to promote peritumoral lymphangiogenesis. These data indicated that lymph node metastasis is promoted by COX-2 and VEGF-C in OSCC. (C) 2013 American Association of Oral and Maxillofacial Surgeons